FastQCFastQC Report
Fri 7 Jul 2023
SRR6939950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19988597
Total Bases614.6 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATCGTATAGTGGTTAGTACTCTGCGT9998205.0019518628546065No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT8378544.1916598748776615No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG4040772.0215375796510378No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTT3924321.96327936372923No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA3895171.948696049052367No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG3880011.941111724849923No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG3007771.5047429291810726No Hit
TTGGTCTAGGGGTATGATTCTCGCTT2933741.4677068130394544No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTT2775231.388406600023003No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2713081.3573138725044085No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT2633201.317351087722665No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA2562761.2821109955841323No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT2147161.0741924508258383No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA2018721.009935814904868No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCT1530090.7654814392425842No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1517790.7593279308197569No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1514400.7576319638641972No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1503750.7523039260834564No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGA1346470.6736190639092878No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT1321680.6612169928684839No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG1243460.6220846815812036No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA1169880.5852736937965181No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG1142550.5716008982521384No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG1022540.5115616668843742No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC982780.4916703258362755No Hit
TTTGGTCTAGGGGTATGATTCTCGCTT960970.4807591048036038No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGTT838050.41926404339434126No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA773860.38715073399098493No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG771360.3859000208969144No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG742080.3712516691391597No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG722090.3612509672389713No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGA673260.3368220390855846No Hit
CAAAGCACCCAACTTACACTTAGGAGATT663560.3319692722805908No Hit
CCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG648310.32433992240676024No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG635080.31772114871293866No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG631220.3157900476956937No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC606680.30351304796429684No Hit
TGGTCTAGGGGTATGATTCTCGCTT589790.295063230300756No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC556120.2782186263498133No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA537940.26912344072973204No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG520880.2605885745757944No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGTT482930.2416027498078029No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACG472930.2365998974315206No Hit
GTGATCGTATAGTGGTTAGTACTCTGCG461560.23091165427968754No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCG454570.2274146604686662No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG437320.21878474011957916No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC416960.20859893268146834No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGT388560.1943908319328265No Hit
TGTGATCGTATAGTGGTTAGTACTCTGCGT387170.19369543545252327No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG377740.188977745661689No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGT370260.18523561208422984No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGG362930.18156852129241485No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGC323730.16195733997738812No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGC323150.16166717453956372No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACGG318200.15919076261330398No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA299200.14968534309836753No Hit
TGGTCGCTGGTTCGTTTCCGGCTCGAAGGA290740.14545292998803266No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGA288980.144572427969807No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG286140.1431516178949428No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG282810.14148566805364077No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGAGA270900.13552727087348854No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA270500.13532715677843724No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCG266990.13357115559436214No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGG266420.13328599300891403No Hit
TTGATCGTATAGTGGTTAGTACTCTGCGT251740.12594180572053157No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG247390.12376556493684875No Hit
ACACAAAGCACCCAACTTACACTTAGGAGATT246410.12327528540397308No Hit
TCGCAGCAACCTCGGTTCGTATCCGAGTCACGG241270.12070381928256395No Hit
CCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG237980.11905788085076706No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG237880.11900785232700423No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGA232490.11631131489618807No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACG231390.115761001134797No Hit
CCGTGATCGTATAGTGGTTAGTACTCTG226690.11340966051794431No Hit
TTTGTCTAGGGGTATGATTCTCGCTT223570.11184877057654422No Hit
TAGTTCAACTTTAAATTTGCCCACAGAACCC215050.10758634035195166No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTT214050.10708605511432343No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGA212540.10633062440550481No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGT212410.10626558732461312No Hit
GCAGCAACCTCGGTTCGTATCCGAGTCACGG211260.10569025930134066No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA210520.10532004822549577No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA208380.10424943781697134No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG207720.10391924956013672No Hit
TTTTGTCTAGGGGTATGATTCTCGCTT206690.10340395576537963No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA206130.10312379603230781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)