FastQCFastQC Report
Fri 7 Jul 2023
SRR6939955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37137187
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length25-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATCGTATAGTGGTTAGTACTCTGCGT27667077.449963832748022No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT25405796.841064725769348No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT7738242.083690399060112No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG4436871.1947243069325633No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG4057551.0925840990595221No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTT3824151.0297360432818996No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT3791491.0209416238230429No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTT3661770.9860116761132177No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA3477770.9364656509928984No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG3109280.837241657533189No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG2838460.7643174481685971No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT2618610.7051180263060851No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA2371330.6385324768943862No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2316000.6236336640144554No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG2283070.6147665411491721No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA2141580.5766672634629004No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1959090.5275278388748185No Hit
TAGTTCAACTTTAAATTTGCCCACAGAACCC1651430.44468365361113643No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA1591780.4286215862283807No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG1518940.40900782280574993No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG1265790.3408416474839626No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC1245530.33538619928321445No Hit
TGTGATCGTATAGTGGTTAGTACTCTGCGT1107830.29830746200567104No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGTT1003300.2701604728435678No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA984870.2651977921752663No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG966760.26032127850717396No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA907360.2443265290933317No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACG904380.24352409890388305No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG889450.23950386980037017No Hit
CCGTGATCGTATAGTGGTTAGTACTCTG856080.2305182673097992No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC813990.21918461406352613No Hit
TAGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT803870.21645958268190854No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACAC798810.21509706699109976No Hit
TGCAGCTCAAAACGCTTAGCCTAGCCACAC779470.20988934891595315No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACGG765890.20623263684457308No Hit
TCATCACCTCTAGCATCACCAGTATTAGAGGCA733790.19758900963608256No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGG713600.19215241046663012No Hit
TTGATCGTATAGTGGTTAGTACTCTGCGT666000.17933506918550401No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG648950.17474398370560484No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG593230.15974015479416898No Hit
TGATCGTATAGTGGTTAGTACTCTGCGT591520.1592796998868008No Hit
GTGATCGTATAGTGGTTAGTACTCTGCG582160.1567593151306802No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC568550.15309452490303047No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG563250.15166738396206475No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCAC562810.15154890433677703No Hit
TCGCAGCAACCTCGGTTCGTATCCGAGTCACGG545580.1469093499192602No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG529470.14257138000247568No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGTT517610.1393778155572203No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGC499360.13446360382653647No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACG483810.13027642616011817No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA483110.1300879358471604No Hit
TAGTTCAACTTTAAATTTGCCCACAGAACC479760.1291858750637198No Hit
GCAGCAACCTCGGTTCGTATCCGAGTCACGG468640.12619157180644835No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC460790.12407778758256516No Hit
ATCGTATAGTGGTTAGTACTCTGCGT445650.12000101138516495No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCC440060.1184957816002596No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGT436510.11753986644168822No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG413760.11141393127056178No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTG375610.10114120921436512No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAA373290.10051649846284803No Hit
TGCAGCAACCTCGGTTCGAATCCGAGTCACGG373190.10048957127528264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)