FastQCFastQC Report
Fri 7 Jul 2023
SRR6939959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30706122
Total Bases946.9 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATCGTATAGTGGTTAGTACTCTGCGT13501394.396970089547615No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTT8600822.8010114725656337No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA8547942.7837901510324228No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT8361682.7231312374776597No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG5856461.9072613598031036No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA5669461.846361451960622No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTT4978751.6214193378115285No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG4505421.4672709240196466No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT3475651.1319078325813987No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA2688690.8756201776310275No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG2275850.7411714185203849No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGTT2043370.6654601320218815No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT1907950.6213581773693206No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG1720660.5603638258194897No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG1683920.5483987851022021No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1187580.3867567516340878No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGTT1113060.36248797552488066No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT1030230.3355128986981814No Hit
ATCGTATAGTGGTTAGTACTCTGCGT969210.31564064000006253No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC949470.3092119545411824No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC929880.30283211927575876No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG929810.3028093225188124No Hit
TCGACTGCATAATTTGTGGTAGTGGGG784950.25563306235805355No Hit
TGTGATCGTATAGTGGTTAGTACTCTGCGT747550.24345308078955719No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGA721490.234966173846375No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG614680.2001815794257575No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA607750.19792470048806554No Hit
ATCGTATAGTGGTTAGTACTCTGCGTT606450.19750133214477555No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG597140.19446936347090654No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG541690.176411075289807No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACG541560.176368738455478No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGG478250.155750700137256No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC442560.14412761077416417No Hit
TGTGATCGTATAGTGGTTAGTACTCTGCGTT437160.1423690038097289No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG433370.1411347222550604No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGA432710.14091978140385167No Hit
CGACTGCATAATTTGTGGTAGTGGGGG408780.13312654720775224No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGT385460.12553197046504275No Hit
TTGATCGTATAGTGGTTAGTACTCTGCGT382030.12441492937466997No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG359600.11711019711313594No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA358170.11664449193551697No Hit
GTGATCGTATAGTGGTTAGTACTCTGCG332570.10830739225226813No Hit
CCGTGATCGTATAGTGGTTAGTACTCTG328480.10697541031068657No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACG319640.10409650557631472No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACGG318530.10373501414473633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)