FastQCFastQC Report
Wed 5 Jul 2023
SRR6939963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10602937
Total Bases323.3 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATCGTATAGTGGTTAGTACTCTGCGT1698161.6015939734433957No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1668841.573941257974088No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1211381.1424947634792133No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1016500.9586966328291868No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA590430.5568551430608331No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG579970.5469899519350158No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCT553540.52206289634655No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG543620.5127069980704403No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT469060.4423868594145188No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC404650.38163954006328626No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA401500.37866866510665864No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG380520.35888169476061205No Hit
ATCGTATAGTGGTTAGTACTCTGCGT265510.25041174912196495No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGC237150.22366444316324804No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC225110.21230909888458263No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG223920.21118676834541222No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCC210420.1984544471027226No Hit
ACAAAGCACCCAACTTACACTTAGGAGATT199980.1886081186750426No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGA194080.18304362272453378No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA193270.1822796834499724No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT191210.18033682554182864No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTT182010.17165998439866237No Hit
AAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCT181880.1715373768607698No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCA175640.1656522150419266No Hit
AGAAATAAGGCCTACTTCACAAAGCGCCTTCCC174190.16428466942697104No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCC173870.16398286625677394No Hit
TGAAATAAGGCCTACTTCACAAAGCGCCTTCC171260.16152128414985395No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA170090.16041781630882085No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG153390.1446674633641603No Hit
TTTGAGGCCTTGTTCCGACAAGGCT152820.14412987646724676No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC147680.13928216304595606No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG143550.13538701588059987No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC142650.13453819446442056No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC131100.12364498629011943No Hit
TGAAATAAGGCCTACTTCACAAAGCGCCTTCCC121330.1144305582500396No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATT120330.1134874233431737No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG112980.10655538177770933No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGAA108760.10257535247073524No Hit
TTTTGAGGCCTTGTTCCGACAAGGCT107210.10111349336509308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)