FastQCFastQC Report
Fri 7 Jul 2023
SRR6949916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6949916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40765023
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG23630935.796864140123262TruSeq Adapter, Index 2 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT8212152.0145088597153493No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3919950.9615964156330784Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC3774980.9260340660178211Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT3242250.7953509556464619Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT3095670.7593936596086307No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA3080800.7557459246374031Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2662080.6530304177677024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC2622990.6434413148742735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC2423070.5943992721407271No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2405530.5900965639097028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG2383850.5847782791634878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG2109770.5175441701578336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC2052770.5035615949486892No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT2024150.4965408703436767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT1903650.46698121573487156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT1885420.46250924475131533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1719580.4218273101428153No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC1613280.39575103391944605No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1604760.3936610068881845No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1536600.3769407906380919No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT1375810.33749766313145463No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1235720.3031324182007698Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1219690.2992001255586192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTC1211240.2971272701109478No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1170560.2871481269616848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1131340.27752713398444545No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTC1124490.2758467718759781No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1113510.2731532863356903No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1097330.2691841974429893No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1080950.2651660468828878Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT1061080.26029177022664746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGT1009650.24767556245460723No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC978230.2399679745059876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT970700.23812080272835856No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG845020.20729045093388024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT806680.19788532929320315No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC786790.19300614647022277No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC747260.18330910790851265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC732650.17972515310490564No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT698170.1712669216450583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTGA674440.1654457548079882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC660100.1619280332553719Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTAGATCGGAAGAGCACAC644400.15807669236443214No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA618780.15179189277042723Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA567120.1391192640808764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTC560690.1375419314739501No Hit
TTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAA542520.13308467899061407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGACCGGCGCAAGACGGACCAGAGCGAAAGAGATCGGAAGAGCACACGTC531140.13029307011552527No Hit
CTAAACCATTCGTAGACGACCTGCTAGATCGGAAGAGCACACGTCTGAAC523020.1283011664190647Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC497430.12202372607516987TruSeq Adapter, Index 2 (100% over 50bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC471570.11568005248028439Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT464470.1139383632875664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG449920.11036912698417956No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG440520.10806322861635574No Hit
GGACCGGCGCAAGACGGACCAGAGCGAAGATCGGAAGAGCACACGTCTGA438360.10753336260843026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA437450.10731013202175796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA435310.10678517218057255Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTCTGA417100.10231810736375643Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC414740.10173917968843045Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)