FastQCFastQC Report
Fri 7 Jul 2023
SRR6949924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6949924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25368437
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2847561.1224814520500415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT2540721.0015280011141403No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1884410.742816752959593No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1607210.633547112106276No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1343860.5297370113893891No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1302280.5133465652613916Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1104840.43551756854393514No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1088020.4288872822555051Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1058740.4173453807974059No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC1039170.4096310702941612No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1027390.4049875047485188No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT954040.37607362250973525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA944820.3724391849604294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT937600.36959312865826144No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT819020.3228500045154536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG793820.31291640080151567TruSeq Adapter, Index 12 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG730220.2878458771425295No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA717910.28299339056639555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG715070.2818738891954597Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG686890.27076559742328626No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA666900.26288572685814265No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT602140.2373579420758165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG592750.2336564921205039No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG590290.23268678318652428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC579960.22861479404505686Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA524330.20668596965591535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC520650.20523534816118152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA518690.20446273453898636No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC512960.2022040222659362No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC511590.2016639811116467No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA498820.19663016684867107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC494320.1948563090426107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT492360.19408369542041554No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCT474440.18701979944605968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC471630.18591212379383087No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC463690.18278225024269332Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT452060.17819781329058626No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT440940.17381441355649935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA440520.1736488534946004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC430850.1698370301646885No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG430310.16962416722796125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC428640.1689658688866011No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTC421170.1660212649285409No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT383930.15134160610683267No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT376880.14856256221067146No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC363650.14334742026085406No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT351420.13852646893460563No Hit
CTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGTCTGAA349970.1379548925304306Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA349250.13767107528146097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT349240.1376671333752253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG339720.13391443863884875No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA335330.132183941801381Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA326790.12881755387610203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC315130.12422129120528788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT306120.12066963368693151No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT302780.11935303700421117No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT301440.11882482156862877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA295170.11635324635885136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG292650.11535988598745757No Hit
AACCGGGGTTCGAATCCCCGTAGGGACGCCAAGATCGGAAGAGCACACGT289140.1139762768987305No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG287800.11344806146314809Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA282950.11153623693883861No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA277390.10934453707179516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC272330.1073499325165362No Hit
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT271860.10716466292345879No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTC270890.10678229801859689No Hit
TTGGTGACTCTAGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTG267760.10554848136682604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCT258480.10189039238010604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)