FastQCFastQC Report
Mon 10 Jul 2023
SRR7074420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16126418
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA810607350.26579988190806Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA7025064.356243277335364No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA6940534.30382618136278No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT5668183.5148412995371943No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG3170371.9659480487235295No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA2324321.441312013616415No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1494690.9268580288567493No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT1438620.8920889933523986No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1119860.6944257553041228Illumina RNA PCR Primer (100% over 46bp)
ATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT933460.5788390205438058Illumina RNA PCR Primer (97% over 46bp)
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA878170.5445536634359843Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA749850.46498236620184347Illumina RNA PCR Primer (97% over 45bp)
TTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA725460.44985811480267973No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC644200.3994687474924686No Hit
ATCGTCTGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTAT481200.2983923646280284Illumina RNA PCR Primer (100% over 31bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC462430.2867530780859085No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC458510.2843222840930949No Hit
GATCGTCGGACTGTAGAAATCTGAACGTGTAGATCTCGGTGGTCGCCGTA444800.2758207061233313Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA437960.27157921864607504No Hit
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA408960.25359630390332183Illumina RNA PCR Primer (97% over 45bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA404590.2508864646817415Illumina RNA PCR Primer (97% over 45bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT404570.2508740626715741No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC396570.2459132586046077Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC341660.2118635396899671No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC319640.19820892649564212No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG306410.19000499676989646No Hit
CCCGGGTCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT299230.18555267511979412No Hit
AGCGACTCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT267290.1657466648824308No Hit
GATCGTCGGACTGTATAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA231280.14341684557599835Illumina RNA PCR Primer (97% over 45bp)
GAGCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA227410.14101705660860334Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGATGGTCGCCGTA217400.13480985051981165Illumina RNA PCR Primer (97% over 45bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAGATCGTCGGACTG211810.13134348867801887No Hit
GATCGTCAAACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA207560.128708061517443Illumina RNA PCR Primer (100% over 41bp)
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG201870.12517968962481313Illumina RNA PCR Primer (100% over 33bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGA198790.1232697800590311No Hit
GATCGTCGAACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA195990.12153349863559286Illumina RNA PCR Primer (97% over 45bp)
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA195280.12109322727464958No Hit
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC185540.11505344832311801No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGT182000.11285829252348538No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTAGTCGCCGTA178350.11059492566793197Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGAGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA171810.10653946834318694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)