FastQCFastQC Report
Fri 7 Jul 2023
SRR7074421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19890998
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA963461848.4370769128829Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA10041975.048499828917584No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA8839334.443884615543172No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT5930892.9816955388563207No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG3835641.9283295890935186No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT2188841.1004173847888377No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1884560.9474436627061146No Hit
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1444150.7260319467127794Illumina RNA PCR Primer (100% over 45bp)
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1316640.6619275714571988Illumina RNA PCR Primer (97% over 45bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1272150.6395606696054165Illumina RNA PCR Primer (100% over 46bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA916370.46069583838880285Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC766860.3855311835032108No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA703060.3534563725761774Illumina RNA PCR Primer (97% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC645860.32469964553814745No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA535590.2692625075926306No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT531490.267201273661583No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA512470.25763915918145486No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC507830.2553064456594888No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGGATCGTCGGACTGTAGA440020.22121564739989416No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC439680.22104471580561216No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG408120.20517824193637743No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC401400.2017998292493921No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC365710.18385703924961433Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT362420.18220302470494443Illumina RNA PCR Primer (100% over 46bp)
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG338580.1702177035058774Illumina RNA PCR Primer (100% over 33bp)
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA311780.1567442719565906No Hit
GATCGTCGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA307070.1543763666358018Illumina RNA PCR Primer (97% over 45bp)
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGT279460.1404957157001373No Hit
GATCGTCGGACTGTAGAAATCTGAACGTGTAGATCTCGGTGGTCGCCGTA257110.12925947707601199Illumina RNA PCR Primer (97% over 45bp)
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC253040.12721332534445984No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGATGGTCGCCGTA247130.12424213204385219Illumina RNA PCR Primer (97% over 45bp)
ATTTAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG242670.12199991171885896No Hit
GATCGTCGGACTGTAGAACTCTGGACGTGTAGATCTCGGTGGTCGCCGTA237770.11953648580126548Illumina RNA PCR Primer (97% over 45bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAGATCGTCGGACTG235110.11819919744600045No Hit
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA230760.11601227851915726Illumina RNA PCR Primer (100% over 45bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGA224990.11311146881619513No Hit
TTCAGCGAGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA213150.1071590274153162No Hit
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA212100.10663115043297476No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGTACTGTAGAA210390.10577146506173295No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGACCT201530.10131718881073741No Hit
TCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGATCGTCGG200370.10073401043024588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)