FastQCFastQC Report
Mon 17 Jul 2023
SRR7074422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23401879
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA436766018.663714994851482Illumina RNA PCR Primer (100% over 45bp)
GATCGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA428851818.325528475726244Illumina RNA PCR Primer (97% over 45bp)
GATTGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA10713044.5778546244085785Illumina RNA PCR Primer (97% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA6965202.9763421988465115No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA6675582.852582905842732No Hit
GATCGACTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA6076082.5964068953608384Illumina RNA PCR Primer (100% over 42bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT5402782.308694955648647No Hit
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4056691.7334890074425222Illumina RNA PCR Primer (100% over 45bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3498471.4949526061561125Illumina RNA PCR Primer (97% over 45bp)
GATCGACAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2641431.1287256036149917Illumina RNA PCR Primer (100% over 42bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG2346881.0028596421680498No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA2191940.9366512834289931No Hit
GATTGACTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1921840.8212332009750156Illumina RNA PCR Primer (100% over 42bp)
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1751680.7485210909773528No Hit
TCAGCAGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1630960.6969354896673041No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT1322940.5653135801616613No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1197190.5115785787970274No Hit
ATTTAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1015290.43384977761828436No Hit
GATCGACGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA978990.4183382026716744Illumina RNA PCR Primer (100% over 30bp)
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT735810.3144234700128139No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC689480.2946259144404601No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT663090.283349042185886Illumina RNA PCR Primer (100% over 46bp)
TTCAGCGAGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA536290.22916535890130874No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA530730.22678948130618057Illumina RNA PCR Primer (97% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC486280.20779528002858233No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC485240.20735087126978138No Hit
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG477900.20421437099132084No Hit
TCAGCAGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA475490.20318453915602247No Hit
CCCGGAGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT473340.20226580951042436No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT467240.1996591812136111No Hit
AGCGAAGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT452630.19341609278468622No Hit
GATTGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA438650.1874422135077273Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA427130.18251953187177833No Hit
AATTCAGCGGGTCGCCACGACTGATCTGAGGTCGCGATCGTCGGACTGTA417490.17840020453058492No Hit
GATCGACGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA398850.17043503216130634Illumina DpnII expression Sequencing Primer (96% over 26bp)
GATCGACTGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA365270.15608575704540648Illumina RNA PCR Primer (97% over 42bp)
GATCGTCGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA342040.1461592037118045Illumina RNA PCR Primer (97% over 45bp)
GATTGACGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA339050.14488152853025177Illumina RNA PCR Primer (100% over 30bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC322890.1379761001242678No Hit
TTCAGAGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA317670.1357455100079784No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA315410.1347797755898148Illumina RNA PCR Primer (97% over 45bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG297410.12708808553364453No Hit
ATTTAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG293970.12561811810068757No Hit
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGGATCGTCGGACTGTAGAAC288140.12312686515471684No Hit
ATCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT282820.1208535434270043Illumina RNA PCR Primer (100% over 46bp)
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA282370.12066125117560003No Hit
ATTCAACGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG271970.11621716358759056No Hit
GATCGACAAACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA266110.11371309115819289Illumina RNA PCR Primer (97% over 42bp)
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC243360.10399164955942214No Hit
ATCTTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT241860.1033506753880746Illumina RNA PCR Primer (97% over 46bp)
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC238810.10204736123966798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)