FastQCFastQC Report
Fri 7 Jul 2023
SRR7074423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44798181
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA952941921.27188824921262Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA834010218.617055009443355Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA22640785.053950739651683No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT17423443.889318631039952No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA16825123.755759636758466No Hit
GAGTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA12802932.857912913919429Illumina RNA PCR Primer (100% over 45bp)
GAGCGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5954681.329223612896247Illumina RNA PCR Primer (97% over 45bp)
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4922121.098732111466758Illumina RNA PCR Primer (100% over 45bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4867541.0865485810685036Illumina RNA PCR Primer (97% over 45bp)
GAGCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4850781.0828073577362438Illumina RNA PCR Primer (97% over 45bp)
GATCGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4222690.9426030043496632Illumina RNA PCR Primer (97% over 45bp)
ATGCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG4136590.9233834739852496No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT4097300.9146130285959602No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA3282000.7326190320093577No Hit
AAGTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA2519920.5625049820661245No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA2340870.5225368413954129No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC2143550.47849041013517934No Hit
ATGTAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG2103440.4695369215995623No Hit
GAGTGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1974530.440761199656745Illumina RNA PCR Primer (97% over 45bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1860460.41529811221576163No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1805140.402949396539114No Hit
TTGAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA1483430.33113621287435757No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1482090.3308370935864561Illumina RNA PCR Primer (100% over 46bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC1461700.3262855694966722No Hit
GATCGACAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1377410.3074700733942747Illumina RNA PCR Primer (100% over 42bp)
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1328050.29645176887874086No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC1077430.2405075331072036Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC1034520.230929019193882No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA1016050.22680608393452406No Hit
GAGTGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA898660.20060189497426245Illumina RNA PCR Primer (97% over 45bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC888060.19823572747295254No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA869630.19412172114756177Illumina RNA PCR Primer (97% over 45bp)
CCGGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT822740.18365477830450302No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT753590.16821888370869342No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG687070.15337006652122773No Hit
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC662760.14794350690265748No Hit
GAGCGACTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA657840.146845248024691Illumina RNA PCR Primer (100% over 42bp)
AGGGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT631940.1410637632809243No Hit
GATCGACTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA594060.132608062813979Illumina RNA PCR Primer (100% over 42bp)
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG566110.1263689702043929Illumina RNA PCR Primer (100% over 33bp)
ATCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT559560.12490685726726271Illumina RNA PCR Primer (100% over 46bp)
ATGGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT545880.12185316185047781Illumina RNA PCR Primer (100% over 46bp)
ATGCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG506970.11316754133387692No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC496030.11072547789384574No Hit
ATTTAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG472740.1055266060914393No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGACCT472280.10542392335081642No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAGATCGTCGGACTG460130.1027117596582772No Hit
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT455170.1016045718463435No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGT454440.10144161880144195No Hit
ATGTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT449280.10028978631967223Illumina RNA PCR Primer (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)