FastQCFastQC Report
Wed 5 Jul 2023
SRR7074424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18903331
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA633726133.524572997214094Illumina RNA PCR Primer (100% over 45bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA274365614.51414039144741Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT4059722.1476214959151907No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA3899312.062763435714055No Hit
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3778401.9988011636679273Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA3487681.8450081628470665No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA3021271.5982738703564998No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA2612841.3822114208337144No Hit
GATTGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2271261.2015131089859241Illumina RNA PCR Primer (97% over 45bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1393020.7369177421693563No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1366380.7228249878288646No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGGATCGTCGGACTGTAGA1262820.6680409923520887No Hit
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1197300.6333804343795282No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1112500.5885206157581434No Hit
TCAGCGTTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1070800.5664610115539954No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT884370.4678381815353072No Hit
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA832390.44034038233790646Illumina RNA PCR Primer (97% over 45bp)
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA626220.33127494831466475Illumina RNA PCR Primer (97% over 45bp)
TTCAGCTTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA599250.3170076215668022No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC552730.29239820220044815No Hit
TTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA474400.25096106077812425No Hit
GATCGTTTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA458130.24235411208744112Illumina RNA PCR Primer (100% over 42bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT413480.21873393636285582No Hit
GATCGTCTGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA384670.20349323619207643Illumina RNA PCR Primer (100% over 30bp)
ATTTAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG349360.18481398860338424No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT335840.17766180997412573Illumina RNA PCR Primer (100% over 46bp)
GATCGTCGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA324580.17170518783171074Illumina RNA PCR Primer (97% over 45bp)
GCACGTCAGGACCGCTACGGACCTCCACCAGAGATCGTCGGACTGTAGAA315070.16667432845565686No Hit
ATCTTCTTACTGTAGAACTCTGAGCGTGTAGATCTCGGTGGTCGCCGTAT307540.16269090352382867Illumina RNA PCR Primer (97% over 42bp)
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT306190.16197674367549295No Hit
ATCTTCTTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT304730.16120439302470024Illumina RNA PCR Primer (100% over 42bp)
ATTTAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG301740.15962266121246038No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC288480.15260802447991836No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC259410.13722978241242245No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA254490.13462706652071002Illumina RNA PCR Primer (97% over 45bp)
AGCGACTCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT251730.13316700638633477No Hit
CCCGGGTCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT249150.13180216756507093No Hit
ATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT242990.12854348262747978Illumina RNA PCR Primer (97% over 46bp)
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA240390.1271680636603147No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGGATCGTCGGACTGTA223410.11818551979013647No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG217210.11490567456074277No Hit
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC206870.10943573912978617No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA205010.10845178556096807No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC197140.10428849814881833Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG196840.10412979596029928Illumina RNA PCR Primer (100% over 33bp)
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGT195570.10345795669556862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)