FastQCFastQC Report
Mon 14 Aug 2023
SRR7074425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23916331
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1319171655.157774827585385Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA8499923.5540233993249215No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA7916213.309960043620403No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT4688201.9602505083242077No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG3465041.4488175464706523No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT2416781.0105145308450532Illumina RNA PCR Primer (100% over 46bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2379730.9950230242255805Illumina RNA PCR Primer (97% over 45bp)
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1652550.6909713701487072No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT1554780.6500913538953781No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1171110.48966959020595596Illumina RNA PCR Primer (97% over 45bp)
GCACGTCAGGACCGCTACGGACCTCCACCAGAGGATCGTCGGACTGTAGA898170.37554673415416434No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA833110.3483435649055033No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA810870.3390444796904676Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC777740.32519202046501194No Hit
ATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT752520.3146469247310551Illumina RNA PCR Primer (97% over 46bp)
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA715440.29914287438152615Illumina RNA PCR Primer (100% over 45bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT627250.26226848925949386No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC579840.24244521452726175No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA529560.22142192295298138No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA499530.20886564916667194No Hit
GATCGTCGGACTGTAGAAATCTGAACGTGTAGATCTCGGTGGTCGCCGTA450770.18847790658190838Illumina RNA PCR Primer (97% over 45bp)
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC423150.17692931244345128No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGATGGTCGCCGTA416260.17404843577386514Illumina RNA PCR Primer (97% over 45bp)
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC402500.16829504492139702No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC398530.16663509131061952No Hit
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA384090.16059737591020964Illumina RNA PCR Primer (97% over 45bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG338930.1417148809321965No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGGATCGTCGGACTGTA299630.12528259455850482No Hit
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA283650.11860096768187395Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA281250.11759746927737368No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGT264840.1107360489366032No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC264060.11040991195514062Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCCTA254310.10633319968685831Illumina RNA PCR Primer (97% over 45bp)
TTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA248140.10375337253862224No Hit
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT247800.10361121026465138Illumina RNA PCR Primer (100% over 44bp)
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC240290.100471096507236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)