FastQCFastQC Report
Fri 7 Jul 2023
SRR7074426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71225215
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2728072838.302064795452004Illumina RNA PCR Primer (100% over 45bp)
GATCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA66112629.282193110964986Illumina RNA PCR Primer (100% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT27773353.8993704687307713No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA27196963.818445476085962No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA16850912.365862988268972No Hit
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA11980811.6821023285082397Illumina RNA PCR Primer (97% over 45bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG10289981.4447102757078374No Hit
TTCATCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA8151911.1445258536601117No Hit
GATCTTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA6174670.8669219180314164Illumina RNA PCR Primer (97% over 45bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT4088930.5740846131527999Illumina RNA PCR Primer (100% over 46bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC4076280.5723085567379473No Hit
CCCGTGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT3286480.46142086057585086No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT3274090.45968130808731145No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA3078720.4322514154572928No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA3036080.42626477154193215No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA2657090.373054682951817No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2488850.34943383463286143Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA2477680.3478655697985608No Hit
TTCATCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA2033950.2855660035564652No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC1801080.25287112155435404No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT1501700.21083825440189968No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA1461800.2052363057099933Illumina RNA PCR Primer (97% over 45bp)
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC1393710.19567648900743928No Hit
CTTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTG1302550.182877650843174Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1271170.17847190773660704No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC1245530.1748720590032617No Hit
ATCGTCGGACTGTAGAACTCTGAGCGTGTAGATCTCGGTGGTCGCCGTAT1240240.1741293445025052Illumina RNA PCR Primer (97% over 46bp)
ATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1205830.16929819025467313Illumina RNA PCR Primer (97% over 46bp)
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT1193440.1675586377661338No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA1079650.15158255401545648No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGACCT1077840.15132843052843012No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG1075750.15103499512075885No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGGATCGTCGGACTGTAGA1058030.14854711214279942No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC1049310.1473228266141422Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTCGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA949360.13328987494105846Illumina RNA PCR Primer (97% over 45bp)
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA944440.13259910833543992No Hit
GCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGAA939820.13195046164479812Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTGA903080.1267921760573134Illumina DpnII expression Sequencing Primer (100% over 24bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA899920.12634851295289176Illumina RNA PCR Primer (97% over 45bp)
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC879200.12343943082516494No Hit
ACCCTGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC856980.12031974912255441No Hit
CTCCTGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT850030.11934397109225996No Hit
ATCGTCTGACTGTAGAACTCTGAGCGTGTAGATCTCGGTGGTCGCCGTAT831530.11674657633536101Illumina Single End PCR Primer 1 (96% over 28bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC819770.11509547566827283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)