FastQCFastQC Report
Fri 7 Jul 2023
SRR7074427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20276667
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA900786044.42475679065006Illumina RNA PCR Primer (100% over 45bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA9919634.892140310831164Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT7995073.9429902360185722No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA6672483.2907183414315573No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA6302173.108089707248238No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG3033731.496167984610094No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA2284811.1268173413312947No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA2203871.0868995382722417No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT1398090.6895068109566528No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC1370630.6759641513075103No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1344350.6630034413446746No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1306430.6443021429508115Illumina RNA PCR Primer (97% over 45bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT894880.44133486040876446Illumina RNA PCR Primer (100% over 46bp)
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA876340.4321913458459421No Hit
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA801410.39523754076545226Illumina RNA PCR Primer (97% over 45bp)
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG763100.3763439030684876No Hit
TTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA733250.36162254871572336No Hit
ATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT653830.32245437576106567Illumina RNA PCR Primer (97% over 46bp)
TCAGCGTTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA501880.24751602420654242No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT482840.23812592079359No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC463280.22847936497650229No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC444260.21909912511755505No Hit
AGCGACTCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT406050.20025480519061636No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA397640.19610718073142888Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA394790.19470162428568757No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG391940.19329606783994627No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC367030.18101101132646702No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC343280.16929804094528947No Hit
CTTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTG331850.16366101983131648Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC312830.15428077997236922Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCGTCTTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT304650.15024658638424154Illumina RNA PCR Primer (100% over 42bp)
TTCAGCTTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA300440.1481703082661465No Hit
CCCGGGTCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT285520.14081209697826572No Hit
ATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT273280.13477560192708202Illumina RNA PCR Primer (97% over 46bp)
GCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGAA271840.13406542603870744Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG259180.1278217963534145Illumina RNA PCR Primer (100% over 33bp)
GCACGTCAGGACCGCTACGGACCTCCACCAGAGGATCGTCGGACTGTAGA242990.11983724938620335No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGATC234670.11573401092003928No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC233930.1153690594218468No Hit
GCTTTACCGGATAAAACTGCGTACGTGATCGTCGGACTGTAGAACTCTGA230440.11364786924793903Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC224970.11095018722751623No Hit
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA216550.10679763099132612No Hit
TTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTGA211810.10445996869209323Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGATCGTCGGACTGTA210550.1038385647897655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)