FastQCFastQC Report
Fri 7 Jul 2023
SRR7074428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19982941
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA348447517.43724810076755Illumina RNA PCR Primer (100% over 45bp)
GAGCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA18197759.106642510729527Illumina RNA PCR Primer (100% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT9029844.518774288529402No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA8260524.133785912694233No Hit
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA7985153.9959833740188695Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA5157432.5809163926370995No Hit
GATCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4564242.2840681959677505Illumina RNA PCR Primer (100% over 45bp)
GAGCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4563102.283497709371208Illumina RNA PCR Primer (97% over 45bp)
GAGCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3821551.9124061868570796Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA3523271.7631388692985681No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA3291731.6472700389797479No Hit
GATCTTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2083551.0426643405492715Illumina RNA PCR Primer (97% over 45bp)
GAGCTTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1713990.8577265979016803Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC1675950.8386903609433666No Hit
ATGCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1651930.8266701082688479No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1554100.7777133506023963No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT1488970.7451205505736118No Hit
TTCATCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA1429810.7155152987740894No Hit
TTCATCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA1323820.6624750581008071No Hit
CCCGTGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT999870.5003617835833074No Hit
ATGCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG961860.48134055943016596No Hit
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA902780.45177534177776935No Hit
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG776810.3887365728598208No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA735620.36812399135842916No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT618210.30936887618294023No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA540760.2706108174967839Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT531960.2662070613129469No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT530540.2654964552014641Illumina RNA PCR Primer (100% over 46bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC524370.2624088216043875No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG489850.24513408711960868No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC473530.2369671211059473No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC445490.2229351525383576No Hit
TCAGCGTTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA429680.21502340421262317No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC420960.21065968217591197No Hit
AAGTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA402090.201216627722616No Hit
ATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT384010.19216891047218726Illumina RNA PCR Primer (97% over 46bp)
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA375330.18782520550903892No Hit
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGGATCGTCGGACTGTAGAAC352010.17615525162187087No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC346240.1732677887604232Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGATC334290.1672876880335082No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC325000.16263872269852572No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA309190.1547269743727913No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGACCT299820.150037974890683No Hit
CCCGTGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC248210.12421094572615712No Hit
CTCCTGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT246070.12314003229054221No Hit
CGCTACCGGCCTCACACCGTCCACGGGCTGATCGTCGGACTGTAGAACTC235030.11761531998718308Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC228960.11457772907401367No Hit
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA205480.10282770689259405No Hit
ACCCGGGTCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC203720.10194695565582663No Hit
TCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTGAA203360.10176680199376058Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTGA202370.10127137942307891Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCCTTCACCTTCATTGCGCCACGGGATCGTCGGACTGTAGAACTCTGAA201520.10084601660986739Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)