FastQCFastQC Report
Fri 7 Jul 2023
SRR7074429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22727328
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA487836921.46477139767596Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA14230876.2615675718676655No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT14178846.238674427543792No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA11436325.031968562252456No Hit
GAGCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA10581334.655773877157931Illumina RNA PCR Primer (100% over 45bp)
GATCGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5108412.2476949336059215Illumina RNA PCR Primer (97% over 45bp)
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG3350031.4740096152086157No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT2838271.2488357628314248No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC2400511.0562218312685063No Hit
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2371751.0435674620439324Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA2274881.0009447657023298No Hit
GAGCGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2137200.9403657130305859Illumina RNA PCR Primer (97% over 45bp)
ATGCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG1975450.8691958861156048No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA1647630.7249554369083774No Hit
GATCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1390810.6119549117256546Illumina RNA PCR Primer (100% over 45bp)
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1323060.5821449842233983No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC928760.40865340615491624No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC928400.4084950065401441No Hit
GATCGACAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA900730.3963202361491857Illumina RNA PCR Primer (100% over 42bp)
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA897800.3950310392845125No Hit
TTCATCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA894760.39369344253754773No Hit
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC883550.3887610545331154No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT881850.3880130563522469No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT827990.3643147139866156Illumina RNA PCR Primer (100% over 46bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC755600.33246319144951836No Hit
TTCAGCGAGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA726230.3195404228776916No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG725870.3193820232629194No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA710430.31258843978491446No Hit
CCCGTGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT627280.27600252876185005No Hit
GAGCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA602630.2651565551392579Illumina RNA PCR Primer (97% over 45bp)
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA595120.26185216317553917Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC573400.2522953864176202Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGACTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA528810.23267583413237142Illumina RNA PCR Primer (100% over 42bp)
GAGCTTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA499430.21974866557124534Illumina RNA PCR Primer (100% over 45bp)
ACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTGATCGTCGGA487160.214349878701095No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTGATCGTCGGACTGTAGAAC463990.2041551034947883No Hit
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG457730.2014007101934728No Hit
CGCTACCGGCCTCACACCGTCCACGGGCTGATCGTCGGACTGTAGAACTC451110.19848791727738518Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGATC445410.19597992337682635No Hit
TCAGCAGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA433960.19094193562921255No Hit
CTTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTG397250.17478957491175381Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGACCT392530.17271277996251913No Hit
TCAGCGGATCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA391930.17244878060456556No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAACGATCGTCGGACTGTAGA387540.17051718530220536No Hit
AAGTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA378890.16671119455837485No Hit
TTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTGA375380.1651667983143465Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT361690.15914321296370607No Hit
TTCATCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA349950.15397762552641472No Hit
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC328900.14471564805154394No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGGATCGTCGGACTGTAGA326320.14358045081234364No Hit
GATCTACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA322620.14195245477163Illumina RNA PCR Primer (97% over 45bp)
ATGCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG305910.1346000726526233No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAGATCGTCGGACTG301710.13275207714694837No Hit
GGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGATCGTCGGACTGTA293560.12916608586807918No Hit
TCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGATCGTCGG293250.12902968619980315No Hit
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG287070.12631049281288148Illumina RNA PCR Primer (100% over 33bp)
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGT274840.12092930589992805No Hit
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGGATCGTCGGACTGTAGAAC254260.1118741279221209No Hit
TCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTGAA248270.10923853433188452Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACTC243540.10715733939335059Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGCTACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA236330.10398494710860864Illumina RNA PCR Primer (97% over 45bp)
GACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCGATCGTCGGACTGT234740.1032853488100317No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGTACTGTAGAA228960.10074215499507905No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGGATCGTCGGACTGTAGAA228120.10037255589394407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)