FastQCFastQC Report
Fri 7 Jul 2023
SRR7074430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20606248
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA996002348.334966171425286Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA8208943.983714065753261No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA7263173.5247416220556014No Hit
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA6072352.9468489363032027Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT5694592.7635258975821317No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG3554921.725166075842628No Hit
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1543570.7490786289672918Illumina RNA PCR Primer (100% over 46bp)
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1363000.6614498670500326Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA1216000.5901122805083197No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA1100010.5338235276989776Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC1039990.5046964396429665No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT911770.44247259374923564No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA863460.41902824813134343No Hit
TCAGCGGTTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA760110.36887355718517995No Hit
GATCGTCGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA602460.2923676352919755Illumina RNA PCR Primer (97% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC479180.23254112053780968No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGATGGTCGCCGTA421440.20452049300775182Illumina RNA PCR Primer (97% over 45bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT418250.2029724188508262No Hit
ATTCAGCTGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG407230.19762452630871957No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA407090.19755658575010843No Hit
GAGCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA402140.1951544017134997Illumina RNA PCR Primer (100% over 45bp)
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC359920.17466547039519276No Hit
CTTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTG359330.1743791494696172Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCGTCGGACTGTAGAAATCTGAACGTGTAGATCTCGGTGGTCGCCGTA358620.17403459377951774Illumina RNA PCR Primer (97% over 45bp)
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC350790.1702347753943367No Hit
GGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGATCGTCGGACTGTA335160.1626496973151056No Hit
ATCGTCGGACTGTAGAACTCTGAGCGTGTAGATCTCGGTGGTCGCCGTAT305350.1481832112279732Illumina RNA PCR Primer (97% over 46bp)
ATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT299790.1454850004717016Illumina RNA PCR Primer (97% over 46bp)
GATCGTCGGACTGTAGAACTCTGGACGTGTAGATCTCGGTGGTCGCCGTA280740.13624023160354082Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGAAGCCCCGGGAGATCGTCGGACTGTAGAACT280240.13599758675135815No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG251080.12184653897206323No Hit
CCCGGGGCCGCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG235620.11434396014257424Illumina RNA PCR Primer (100% over 33bp)
TCAGCGTGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA231440.11231544917832688No Hit
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC228980.11112163650558803No Hit
GCTTTACCGGATAAAACTGCGTACGTGATCGTCGGACTGTAGAACTCTGA224410.10890386255663816Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT222760.10810313454443526Illumina RNA PCR Primer (100% over 44bp)
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA211270.10252715584127689Illumina RNA PCR Primer (100% over 45bp)
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAAC209360.10160025250593899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)