FastQCFastQC Report
Fri 7 Jul 2023
SRR7074431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7074431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44526083
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2565679757.62194936392676Illumina RNA PCR Primer (100% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA15764043.540405743752488No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA13807543.1010003731969866No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAACT12040892.704232932414019No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAG6420231.441903164938178No Hit
GATCGTCAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5674251.2743654095959889Illumina RNA PCR Primer (97% over 45bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT4570821.0265488657513395Illumina RNA PCR Primer (100% over 46bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGACT2384300.5354838870511022No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGATCGTCGGACTGTAGAAC2370940.5324833985509122No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTA1615460.3628120623141272No Hit
GATCGACAGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1566320.35177583440250065Illumina RNA PCR Primer (100% over 42bp)
GATCGACGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1553260.3488427221410875Illumina RNA PCR Primer (97% over 45bp)
TTCAGCGAGTCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGA1467630.3296112977195861No Hit
GATCGTCGGACTGTAGAACTCTGAACGCGTAGATCTCGGTGGTCGCCGTA1348280.30280678405958145Illumina RNA PCR Primer (97% over 45bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAAC949060.21314697724477585No Hit
ACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAAGATCGTCGGAC934730.2099286389058746No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGGATCGT894750.20094963215156383No Hit
TCAGCGGATCGCCACGTCTGATCTGAGGTCGCGATCGTCGGACTGTAGAA839710.18858833821066184No Hit
ATCGTCGGACTGTAGAACTCTGAGCGTGTAGATCTCGGTGGTCGCCGTAT821950.1845996648750801Illumina RNA PCR Primer (97% over 46bp)
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGATCGTCGGACTGTAGAAC796020.17877611197014567No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGGATCGTCGGACTGTAGAA793790.17827528192857206No Hit
GATCGTCGGACTGTAGAAATCTGAACGTGTAGATCTCGGTGGTCGCCGTA739250.16602628171896458Illumina RNA PCR Primer (97% over 45bp)
GATCGTCTGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA730420.16404317442430316Illumina RNA PCR Primer (97% over 45bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGATCG657380.14763930615679802No Hit
CTTCCTCGGCCCCGGGATTCGGCGAAAGATCGTCGGACTGTAGAACTCTG643620.14454898267157254Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGATCGTCGGAC624340.1402189363928554No Hit
GGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGATCGTCGGACTGTA546020.12262924632288001No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGATGGTCGCCGTA545090.12242037998267219Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGGACGTGTAGATCTCGGTGGTCGCCGTA538070.12084377599529696Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACACTGAACGTGTAGATCTCGGTGGTCGCCGTA466760.10482844403807089Illumina RNA PCR Primer (97% over 45bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGGATCGTCGGACTGTAGAACTC459480.10319344731042253Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGATC447660.1005388235026198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)