FastQCFastQC Report
Fri 7 Jul 2023
SRR7124025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7124025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53833310
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7039921.3077256442154495No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA4169770.7745706143649722No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA3050850.5667216078669508No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA2964150.5506163377284436No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA2266540.4210292846566559No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA2203780.40937107526919675No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1918290.3563388541406798No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1900760.35308250598003355No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAA1816580.3374453474995314No Hit
TTTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1765820.32801624124542966No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA1696790.3151933254707912No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1692980.3144855852259502No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1609600.29899703362100527No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1582710.2940019850163403No Hit
GGGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAAAAAAAAAA1570670.291765451539205No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAA1538390.2857691641104736No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1488590.27651838610704044No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAC1432620.2661214775758726No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1316070.24447131339313893No Hit
AGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1279820.23773756434445512No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAA1216270.22593260566738327No Hit
GGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA1163360.2161041184352216No Hit
GGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1074750.19964404938132171No Hit
GGGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAAAAAAAAAAAA1073320.1993784145912633No Hit
GGGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAAAAAAAAAAA1062970.19745581313874253No Hit
GGCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAA1060810.1970545745747382No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA1051660.19535488343555318No Hit
TTACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1026950.19076478856678142No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAC1001710.18607624164295303No Hit
GGGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAA991120.18410905812776512No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA986990.1833418751327013No Hit
CGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAA958610.17807004622231107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG927110.17221865049724788No Hit
AATCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA921590.1711932630559035No Hit
CGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA915750.17010843286433625No Hit
TATCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA911680.16935239538493918No Hit
AAGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA898250.16685765746152337No Hit
ACGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA895200.16629109374846168No Hit
TGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA888790.16510038115805994No Hit
TGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA885310.1644539412493863No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA880570.16357344551171013No Hit
ATCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA866100.16088551865007No Hit
GCGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAA823940.15305393630820768No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAC823750.15301864217526287No Hit
GCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA803080.14917901202805475No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAA796390.14793628703120798No Hit
GCGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAAAAAAAAAA754420.14013999882229053No Hit
GGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA741450.13773070985232005No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA734680.1364731241679176No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA727510.13514123504573655No Hit
CGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA701210.13025578401179494No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAC698380.12973008718951148No Hit
GCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAA692550.12864711458388867No Hit
TAGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA667490.12399200420706064No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAC667460.12398643144922725No Hit
AGACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA659400.12248921717798887No Hit
GCGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAA649600.12066878295241365No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAA645040.11982172376173786No Hit
CGCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAA632770.11754246580787991No Hit
TCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA622980.11572388916824919No Hit
GGGGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAA622120.11556413677702522No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAA621900.115523269886247No Hit
AGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA613220.11391088528645181No Hit
AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600860.11161490905909371No Hit
GGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAAAAAAAAAAA576340.10706010832326678No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA569780.10584153194369807No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAC559430.1039189304911773No Hit
CCGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAA556820.10343410055967206No Hit
CCGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAAAAAAAAAA554400.10298456476111166No Hit
GTTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA546210.10146320187259524No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAC538890.10010344896124723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)