FastQCFastQC Report
Fri 7 Jul 2023
SRR7132277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53840664
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG47460688.815025015293273No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC36550136.788573409867308RNA PCR Primer, Index 10 (100% over 50bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTC27787775.161112054635879No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCT19406293.604392769004483No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTGGAATTCTCGG10536061.956896371114591No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA8013641.4883991772464025No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC7730781.435862678068012No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA5920491.0996316835914208No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC5888401.093671504497047No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC5504741.02241309653982No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG5271230.9790425318677347No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCT5023240.932982550140912No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG4247000.788808993886108No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTGGAATTCTCGG3641970.6764348225720247No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC3411510.6336307442270771No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG3073650.5708789178380118No Hit
TAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGG2871790.5333868096426151Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGG2674880.4968140808961791No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA2389130.4437408127061731No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC2199350.4084923618326847No Hit
TATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTGGAATTCTCG2194900.4076658489947301No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2150110.39934685798080055No Hit
TTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGT2100230.3900824848668285No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA2085570.3873596358321287No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC1849090.3434374434906672No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG1711480.3178786948095588No Hit
AGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGA1661980.3086849003199515RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1657050.30776923553543095No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC1634890.30365338733563907No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG1602900.2977117815634666No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA1571730.2919224770333442No Hit
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA1549500.2877936275080114RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC1539270.2858935766468259No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG1531410.28443371352180946No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCTGGAATTCTCGGGTG1495730.27780675216041173No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1446740.2687076816140306No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA1363950.25333082816363484No Hit
AGATAACATGCAGATCGTATGGTCTGGAATTCTCGGGTGCCAAGGAACTC1289130.23943426849267682RNA PCR Primer, Index 1 (100% over 26bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1260710.23415573032308815Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC1213960.22547270219401455No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA1190500.2211154008056067No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGC1102270.20472815862746419No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC1077640.20015354936930196No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1061030.19706852055167817RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA1024240.19023539531384678No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1016950.18888140012537735No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA979980.18201484290758374No Hit
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC975470.18117718607630842RNA PCR Primer, Index 1 (100% over 24bp)
TTGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGG972780.18067756370909543No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC946030.17570920002026721No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC940230.17463194733259604No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTG936390.17391873176006895No Hit
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG932580.1732110881842022No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC915570.17005176607777348No Hit
TGTTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG908950.16882221214805226No Hit
ACTGGAACGTACCTTGAGCATATATGCTGTGACCCGATGGAATTCTCGGG907390.16853246832171312No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGT893430.16593963254242183No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGC865410.16073538766163806No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC844040.15676626870723584RNA PCR Primer, Index 1 (100% over 27bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC837930.1556314387207409No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGT816850.15171618240072224No Hit
GCCTCACCCGGGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA813480.1510902614425409RNA PCR Primer, Index 1 (100% over 32bp)
AAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGGAATTCTCGGG798780.14835998307896053No Hit
CGCTAGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGG787180.14620547770361822No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG773740.1437092231997733Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC771590.14330989677244693RNA PCR Primer, Index 1 (100% over 26bp)
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCATGGAATTCTCGGGT768190.14267840381760521No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG724780.13461572465005262No Hit
AAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC710020.1318743022931515RNA PCR Primer, Index 1 (100% over 33bp)
TTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCG694440.12898057869420035No Hit
AAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGAACTC689540.12807048590634024RNA PCR Primer, Index 1 (100% over 26bp)
AGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGGAATTCTCGGGT684050.12705081051749287No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC678880.12609056975968944No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG666490.12378933513895743No Hit
TTTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGG660080.12259878518585877No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC656460.12192643092217435No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC643530.11952490036155573RNA PCR Primer, Index 10 (100% over 50bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG641000.11905499530986469No Hit
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA637980.11849408097938763RNA PCR Primer, Index 1 (100% over 22bp)
TAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA619020.11497257909003498RNA PCR Primer, Index 1 (100% over 32bp)
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCC617610.1147106952469977No Hit
GTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCG611220.11352385995833929No Hit
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA606540.11265462847932187No Hit
TTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTG605390.11244103527400776No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG603610.11211043013882593Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCT600970.11162009443271353No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC600870.11160152111051232No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTG596940.11087158954800409No Hit
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA592410.1100302180522885No Hit
TGTGTTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTC571690.10618182569219428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571190.10608895908118816No Hit
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC568770.10563948468391846RNA PCR Primer, Index 1 (100% over 24bp)
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG567960.10548904077408852Illumina Small RNA Adapter 2 (100% over 21bp)
TTATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCC553020.10271418643722523No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA553010.1027123291050051RNA PCR Primer, Index 1 (100% over 22bp)
AAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA552250.10257117185627578RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTGGAATTCTCGGG551300.10239472529536411No Hit
GCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGG544470.1011261673890203No Hit
TGTGCTTTTATTAGACTAAAACCAAGCGATCGCAAGATCGTGGAATTCTC542070.10068040765619088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)