FastQCFastQC Report
Fri 7 Jul 2023
SRR7132286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96147759
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1733837518.03305160757829No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA22299302.3192740248891295No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC22155012.304266914843018No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG21047102.1890369800506737No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC17374401.8070519979566035No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC14992251.5592927132082193RNA PCR Primer, Index 11 (100% over 50bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC14433251.5011530325943427No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG13293301.3825907268415898No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG11611861.2077098957657453No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC11610361.2075538858893218No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC10878751.131461628762455No Hit
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA7719990.8029297905944952No Hit
TTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGT7177470.7465041384895929No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC6960550.7239430302270488No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC6882930.7158700391550468No Hit
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA6387260.664317095523776RNA PCR Primer, Index 1 (100% over 22bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG5979130.6218688882805891No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA5663020.588991366923071No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC5611900.5836745503345533No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA5596890.582113411504474No Hit
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA5491780.5711812794305482No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA5308790.5521491145727068No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA5292340.5504382062612608No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGG5179360.5386875423690323No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC5092620.5296660112483745No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG4358450.45330749726574493No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC4205750.43742569184581825No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA4165410.43323006623586513No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG4113120.4277915619437371Illumina Small RNA Adapter 2 (100% over 21bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGT3905050.4061509119520924No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC3738040.38878077231108427No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC3737500.3887246087555717No Hit
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA3658550.38051328892647407No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCC3214670.3343468462951903No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG3213300.33420435727472336No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG3183100.3310633584293941Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC3125800.325103781150011No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA3051090.3173334492382709RNA PCR Primer, Index 1 (100% over 22bp)
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTG2939670.30574503561752286No Hit
TTGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTG2897130.30132059552214835No Hit
TGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAA2868690.2983626482651561RNA PCR Primer, Index 1 (100% over 23bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA2839770.29535477784770836RNA PCR Primer, Index 1 (100% over 22bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG2705110.2813492512082367Illumina Small RNA Adapter 2 (100% over 21bp)
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG2608170.2712668529278982No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2606640.2711077228539461No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGC2604770.2709132305413379No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC2583470.26869789029612223No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA2534120.2635651653617844RNA PCR Primer, Index 1 (100% over 22bp)
TTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTG2527760.2629036834857482No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA2497900.25979804687907493No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG2416280.2513090294699432No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG2335130.24286889515542426No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGC2311640.24042578049063004No Hit
ATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAA2309610.24021464712453675No Hit
TTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCG2293750.23856510269781744No Hit
TTTGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGT2283480.2374969550772369No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG2225450.23146145299132764Illumina Small RNA Adapter 2 (100% over 21bp)
AAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA2211560.23001680153564474RNA PCR Primer, Index 1 (100% over 22bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCA2154960.22413002886525935No Hit
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA2012620.2093257316584987RNA PCR Primer, Index 1 (100% over 22bp)
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGT1993580.20734544629376125No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC1959000.20374889860927492No Hit
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG1847530.19215528465931275Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA1761230.1831795164357393No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC1761230.1831795164357393No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG1752840.1823069011936097Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA1708520.17769732937821253No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG1683830.1751294068122794Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGG1657100.17234931081441016No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCC1645560.17114907483179093No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1636270.17018285366380717No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC1542080.16038647349024535No Hit
TTAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG1515910.15766462117957425Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA1507420.1567816052790164No Hit
TTGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG1493230.1553057518480488No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA1481730.1541096761288009No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCTGGAATTCTCGGGTG1460400.1518912156860567No Hit
ATTGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGT1450900.15090315313537364No Hit
ACTTTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGC1414290.14709547208479398No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGGA1402950.14591603741903125RNA PCR Primer, Index 1 (100% over 22bp)
GATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGG1402310.1458494732050905Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTACATATAAAAGCATTATTTAATACAATGTGGAATTCTCGGGTGCCA1332040.13854093052756436No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC1331200.13845356499676711No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAA1328660.13818938827268973No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCA1327810.13810098267604967No Hit
ATGGATGTGATGCCAATGTAATTTGTAACATAGTTGGAATTCTCGGGTGC1315310.13680090037251935No Hit
ATGGATGTGATGCCAATGTAATTTGTAACTGGAATTCTCGGGTGCCAAGG1305640.13579515670250827Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1269330.13201867762721334No Hit
TACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGC1269100.13199475611282838No Hit
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA1258340.13087564526594947RNA PCR Primer, Index 1 (100% over 22bp)
AAGGGGCAATTTGCATTGTATGGCTTCTAAACCATGGAATTCTCGGGTGC1253730.1303961749124075No Hit
TATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTG1253090.1303296106984667No Hit
TTTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGG1252430.13026096635284032No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1234420.12838780776991382Illumina Small RNA Adapter 2 (100% over 21bp)
TTTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCA1226520.12756615575408262No Hit
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC1226170.12752975344958378RNA PCR Primer, Index 1 (100% over 24bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTC1195790.12437003341908363No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCA1195780.12436899335324081No Hit
GCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA1195680.12435859269481259No Hit
ATGGATGTGATGCCAATGTAATTTGTAACATAGTGGAATTCTCGGGTGCC1171960.1218915565156334No Hit
ATGTGATGCCAATGTAATTTGTAACATAGTTGGAATTCTCGGGTGCCAAG1167340.12141104609624859No Hit
CGCTAGTTACATATAAAAGCATTATTTAATACAATGTGGAATTCTCGGGT1138760.11843853791745682No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1135810.11813171849382365No Hit
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1128690.11739119161373278RNA PCR Primer, Index 1 (100% over 32bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA1122200.11671618888173983No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG1092970.11367607642316446No Hit
TGGAACGTACCTTGAGCATATATGCTGTGACCCGATGGAATTCTCGGGTG1085860.11293658960891642No Hit
TTTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1082750.11261312913179806Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1081530.1124862410989735No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTG1080470.11237599411963413No Hit
ATGGATGTGATGCCAATGTAATTTGTAACATGGAATTCTCGGGTGCCAAG1016780.10575181476668635No Hit
TTTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGT975240.10143138125559432No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCT973620.10126289058905678No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGC970620.1009508708362095No Hit
TATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA967190.10059412825212079No Hit
TGGAACGTACCTTGAGCATATATGCTGTGACCCGTGGAATTCTCGGGTGC966410.10051300311638049No Hit
GCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCC962260.10008137579160842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)