FastQCFastQC Report
Mon 10 Jul 2023
SRR7132287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49283336
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG963844519.55720895192647No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC622177412.624498471450876RNA PCR Primer, Index 11 (100% over 50bp)
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC10453872.1211774300343627No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA6599671.3391280979842761No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC6511731.3212843383816388No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCT6437691.3062610047339327No Hit
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA4980161.010516008900047No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTC4807520.9754859127231159No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC4799860.9739316348227726No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG4593010.9319600442632374No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG3574890.7253750030233344No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC3318840.6734203220333949No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA3105260.6300831583316518No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA2523450.5120290558252794No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC2476260.5024538111624587No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA2235500.45360159872294364No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG2216580.4497625728907637No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGG2093970.42488398106816466No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTGGAATTCTCGG1758280.35676967971486345No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA1639280.3326235870071782No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1472160.29871354487853663RNA PCR Primer, Index 1 (100% over 22bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1411620.28642947384892936No Hit
TTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTG1335990.27108351593731395No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1315650.2669563602593785Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1281470.2600209531270367No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG1258430.25534594492548146No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTGGAATTCTCGG1250060.2536476021022603No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC1241080.2518254851903694No Hit
TAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGG1240730.25175446727064094Illumina Small RNA Adapter 2 (100% over 21bp)
TGTTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1236820.2509610956531027No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC1205170.24453904662622675No Hit
TTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGT1182960.24003245234859913No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1133760.2300493619181948No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA1072230.217564411629927No Hit
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC1056680.2144091869105614RNA PCR Primer, Index 1 (100% over 24bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG1006270.20418057738623863No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC989010.20067837940191385No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA972080.19724314117047595No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT967700.19635440263215947RNA PCR Primer, Index 1 (100% over 25bp)
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA954490.19367398343326434RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGT917760.18622116002861494No Hit
TTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCG904920.18361581691628992No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC892170.1810287355547522No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCTGGAATTCTCGGGTG851140.17270340627915287No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC844950.17144740364166905No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG804020.16314236520027786RNA PCR Primer, Index 11 (100% over 49bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG799560.16223739399459483Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG779780.158223866988225No Hit
TGCTTGGACTACATATGATTGAGGGTTGTATGGAATTCTCGGGTGCCAAG764080.1550382060175472No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC761920.15459992399865138RNA PCR Primer, Index 11 (100% over 50bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG752840.15275751625255238No Hit
TTTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG716580.14540005976868123Illumina Small RNA Adapter 2 (100% over 21bp)
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG716220.14532701276553195No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG709780.1440202830425278No Hit
TGCATGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG707020.14346025601838316No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC704680.14298545049791272No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC702670.14257760473032913No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC683750.13873857889814928No Hit
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC673870.13673384447838516RNA PCR Primer, Index 1 (100% over 24bp)
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA655500.13300641823435005RNA PCR Primer, Index 1 (100% over 22bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG643980.13066891413357246Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC641070.13007845085811562RNA PCR Primer, Index 1 (100% over 27bp)
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC634430.12873113946669518No Hit
TGCTTGGACTACATATGGTTGAGGATTGTATGGAATTCTCGGGTGCCAAG633680.12857895821013415No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA632970.12843489328725638No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGT631510.12813864710781753No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAGCTCGTATGC613900.12456543120376429RNA PCR Primer, Index 11 (98% over 50bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC610220.12381872850490479RNA PCR Primer, Index 1 (100% over 26bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCA596320.12099830254997349No Hit
TTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA583500.11839701760449008RNA PCR Primer, Index 1 (100% over 22bp)
TGGAACGTACCTTGAGCATATATGCTGTGACCCGATGGAATTCTCGGGTG579700.11762596590458083No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC567430.11513628054724218No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA564360.11451335193705231No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTG563700.11437943243127859No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG557150.11305038279064551No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT550990.11180046740342416No Hit
TTTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGT547760.11114507345850128No Hit
TGATTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG544060.11039431259280014No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA530800.10770374797680092No Hit
TGCTTGGGCTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG527340.10700168511319932No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG523020.10612512107540772Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA514330.10436184758272045RNA PCR Primer, Index 1 (100% over 23bp)
ATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAA508940.10326817161890177No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG506780.10282988960000597Illumina Small RNA Adapter 2 (100% over 21bp)
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA503890.10224348449139076No Hit
TTGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG493380.10011091781611536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)