FastQCFastQC Report
Thu 13 Jul 2023
SRR7132288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78056300
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2050169326.26526366225404RNA PCR Primer, Index 3 (100% over 50bp)
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG44557475.7083758774115605No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA7394320.9473059829892013No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG6876780.8810025584097633No Hit
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA5866480.7515703408949694RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC5362110.6869541600101465No Hit
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC4739610.6072040309366444RNA PCR Primer, Index 1 (100% over 24bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG4491660.5754384975972471No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG4482770.5742995760752174No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC4050720.518948502555207No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC3921590.5024053151379198RNA PCR Primer, Index 1 (100% over 26bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT3400890.4356970545618995RNA PCR Primer, Index 1 (100% over 25bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC3281400.4203888731595016RNA PCR Primer, Index 3 (100% over 50bp)
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG3193090.40907524440692167Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC3150600.40363173760478016RNA PCR Primer, Index 1 (100% over 27bp)
AAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA3048200.3905130015130105RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA2972120.38076619055732847No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA2835980.3633249334134464No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG2683420.343780066439224RNA PCR Primer, Index 3 (100% over 49bp)
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA2546420.32622863241019623RNA PCR Primer, Index 1 (100% over 22bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC2538830.32525625734245667RNA PCR Primer, Index 1 (100% over 26bp)
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC2367720.3033349005781724No Hit
TGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAA2332150.2987779333634825RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA2206560.28268826475249276RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC2167070.27762909592178975No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2142320.27445830765742163No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC2121660.27181150016078137RNA PCR Primer, Index 3 (98% over 50bp)
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA2055730.2633650326751332No Hit
AGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC2045690.26207878159738546RNA PCR Primer, Index 1 (100% over 27bp)
ACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT2034080.2605913936479182RNA PCR Primer, Index 3 (100% over 35bp)
AAGGATTGGCTCTGAAGATTGAGTGGAATTCTCGGGTGCCAAGGAACTCC1999690.2561855993686608RNA PCR Primer, Index 1 (100% over 27bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC1959880.25108543448767107No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG1941710.24875762750732486No Hit
AGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGA1830870.23455762058924137RNA PCR Primer, Index 1 (100% over 22bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA1790560.2293933993796785RNA PCR Primer, Index 1 (100% over 22bp)
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA1755220.22486589807613222No Hit
AGTTGAACTTGTGCTTCATACGTGGAATTCTCGGGTGCCAAGGAACTCCA1663410.21310387502354067RNA PCR Primer, Index 1 (100% over 28bp)
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA1620140.20756044034882515No Hit
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG1586620.2032661040812849Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG1554470.199147282154035Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1546870.19817362595972396RNA PCR Primer, Index 1 (100% over 32bp)
AGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAACT1532660.19635314510167662RNA PCR Primer, Index 1 (100% over 25bp)
TTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCCA1524400.1952949345536491No Hit
AGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1517700.19443657975076964RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGGATCTCGTATGC1446750.1853469867262476RNA PCR Primer, Index 3 (98% over 50bp)
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1422550.18224666042330984RNA PCR Primer, Index 3 (100% over 34bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1396150.17886448627465046Illumina Small RNA Adapter 2 (100% over 21bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA1370210.17554124394827836No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA1368110.17527220736827134No Hit
AAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAGG1331850.17062684242015058Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAAGGAAC1299900.16653364302432988RNA PCR Primer, Index 1 (100% over 24bp)
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG1295740.16600069437060172No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG1274770.16331417195024617No Hit
TTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAGGAAC1268260.16248015855222447RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1188690.1522862344231023No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1184420.15173919337708808No Hit
AAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGAA1179870.1511562807870729RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1169690.14985209393732474No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA1144190.14658522118009693RNA PCR Primer, Index 1 (100% over 22bp)
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG1143620.14651219696552362No Hit
TAAACGACAATGGATGTGATGCCAATGTGGAATTCTCGGGTGCCAAGGAA1139030.1459241598692226RNA PCR Primer, Index 1 (100% over 23bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT1119780.14345799121915848RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAGGCATCTCGTATGC1109440.1421333063442669RNA PCR Primer, Index 3 (98% over 50bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1058360.1355893118172396Illumina Small RNA Adapter 2 (100% over 21bp)
AACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCC1038130.13299759276317222RNA PCR Primer, Index 1 (100% over 27bp)
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA1037630.13293353643459913No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC1029930.13194706897457348No Hit
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACTTAGGCATCTCGTATGCC1027420.13162550620513655RNA PCR Primer, Index 3 (97% over 35bp)
ATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAA1014230.12993570025737833No Hit
TGTTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG996760.12769757213703442No Hit
AAATTAGAGTGCTTAAAGCAGGCTTCTGGAATTCTCGGGTGCCAAGGAAC981290.1257156693309829RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA979760.12551965696554923No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGAATCTCGTATGC959280.1228959097471953RNA PCR Primer, Index 3 (98% over 50bp)
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG950240.12173777132659376No Hit
AAGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGG948910.12156738149258933Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG947120.12133805983629764Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC942340.12072568133513888No Hit
TGGAATTCTAGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC931640.11935487590367466RNA PCR Primer, Index 3 (98% over 50bp)
ATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAAGGAA930930.11926391591710085RNA PCR Primer, Index 1 (100% over 23bp)
TGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAAGGAACT906950.11619177439873528RNA PCR Primer, Index 1 (100% over 25bp)
AACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGCCAAG896640.11487093290355808No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA880690.11282753602207637No Hit
TGTATGTAAGCGTATTACCGGTGTGGAATTCTCGGGTGCCAAGGAACTCC859850.11015766824714981RNA PCR Primer, Index 1 (100% over 27bp)
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA848270.10867412367739696No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTTGGAATTCTCGGGTGCCAAGG832770.10668837749163104Illumina Small RNA Adapter 2 (100% over 21bp)
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC820500.10511643518844732RNA PCR Primer, Index 1 (100% over 24bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC808010.10351630810069143No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCC808000.10351502697411995No Hit
TGGAATTCTGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC800660.10257468007066695RNA PCR Primer, Index 3 (100% over 41bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG797130.1021224423909409Illumina Small RNA Adapter 2 (100% over 21bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC794950.10184315679836221RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCAGTCACTTAGGCATCTCGTATGCC789500.10114494281691548TruSeq Adapter, Index 3 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)