FastQCFastQC Report
Mon 10 Jul 2023
SRR7132289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44947356
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG850142418.91418040251355No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC491446310.933820000446746RNA PCR Primer, Index 4 (100% over 50bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC9068602.017604773014902No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA7596591.6901083124889482No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC6115981.3606985024881109No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA4314330.9598629116248796No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG3735790.8311478877645218No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC3624460.8063789113646641No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT3325210.7398010241136319RNA PCR Primer, Index 1 (100% over 25bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA2814240.626119142580934No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC2774850.6173555570209736No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG2749990.6118246421435779No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC2205980.4907919389073742No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG2195000.4883490810894416No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA2125030.4727819807687909No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA2122480.4722146504012383No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2116650.47091757744326496No Hit
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2035810.4529320923793604No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA1940040.43162494363405934No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC1937510.4310620629164483No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC1936140.43075726189544944RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1848380.4112321979517549RNA PCR Primer, Index 1 (100% over 22bp)
TGTTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1815120.4038324300988917No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA1748710.3890573674678439No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC1597720.3554647352338144No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1579390.3513866310623477No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC1552730.34545524769020897No Hit
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1525110.3393102811208739No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG1426820.3174424764829326No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA1321590.29403064331525974No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1260660.2804747847682075RNA PCR Primer, Index 1 (100% over 27bp)
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA1183620.26333473319320494No Hit
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA1133100.25209491744075RNA PCR Primer, Index 1 (100% over 22bp)
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA1126170.25055311373598926RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1082450.24082617896367475No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1070820.23823870752264048Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG1065200.23698835588905387Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG1061870.2362474891737792No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC1028220.22876095314705497RNA PCR Primer, Index 4 (100% over 50bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1021380.22723917286703138Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA1018560.2266117722252673RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC992220.22075158325219396No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG963230.21430181566186005Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA954360.21232839591276514No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC909070.20225216362003587RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC858140.1909211300437783RNA PCR Primer, Index 1 (100% over 24bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT789920.17574337409301674No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC782120.17400801061579685No Hit
TGATTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG780200.17358084422140427No Hit
TGCATGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG742760.16525109953074882No Hit
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG729930.1623966490932192Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG727980.16196280822391423RNA PCR Primer, Index 4 (100% over 49bp)
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC726160.16155789007922958No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC724430.1611729953592821RNA PCR Primer, Index 4 (98% over 50bp)
TGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAA721390.16049664856816048RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA715520.15919067631030398RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA708690.1576711208552512No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA683720.1521157329031768RNA PCR Primer, Index 1 (100% over 22bp)
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC681210.1515573018355073No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC672670.1496573013104486No Hit
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA671680.14943704363833993No Hit
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT653700.14543680834085101RNA PCR Primer, Index 1 (100% over 25bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC649090.14441116402931464No Hit
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT636380.14158341149143455RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA622700.13853985093138738RNA PCR Primer, Index 1 (100% over 22bp)
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC611190.13597907739000265RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG610900.1359145574658496Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGATGTGATGCCAATGTAATTTGTAACTGGAATTCTCGGGTGCCAAGG605490.13471092715664965Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG592010.13171186309601837No Hit
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC588200.13086420478214558RNA PCR Primer, Index 1 (100% over 27bp)
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA585560.13027685098985578RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCCA563480.12536443745434103No Hit
ATGTGATGCCAATGTAATTTGTAACATTGGAATTCTCGGGTGCCAAGGAA551910.12279031496313154RNA PCR Primer, Index 1 (100% over 23bp)
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG551370.12267017441470862Illumina Small RNA Adapter 2 (100% over 21bp)
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA537190.1195153726061217No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG532580.11848972829458534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG525110.11682778404140168No Hit
AGCTGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC522220.11618480962484201No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCAAGGAA521990.11613363865051371RNA PCR Primer, Index 1 (100% over 23bp)
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG520420.11578434113009896No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA512980.11412907135182769No Hit
ACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG511050.1136996801324643RNA PCR Primer, Index 4 (100% over 35bp)
TTATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAA505260.11241150647437416RNA PCR Primer, Index 1 (100% over 23bp)
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG503590.11203996070425143No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCA503190.11195096770541964No Hit
TGCCTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG502750.11185307540670468No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG502290.11175073345804812No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCA500760.11141033523751652No Hit
TGCTTGGACTACATATGATTGAGGGTTGTATGGAATTCTCGGGTGCCAAG500140.11127239608932726No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC495060.11014218500416353No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG494550.110028718930653No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGC492520.10957707946158167No Hit
TGCTTGGGCTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG489140.10882508862145306No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG488250.10862707919905233No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG474380.10554124696456005No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT466190.10371911531347917RNA PCR Primer, Index 3 (100% over 34bp)
TTATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCC464160.10326747584440785No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGG462740.10295155069855498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)