FastQCFastQC Report
Fri 7 Jul 2023
SRR7132290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64927827
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT43888016.759506983038259No Hit
AAGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA37852625.829953311081857No Hit
TAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGT35052595.398700621845853No Hit
TTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGA29630994.563681147068113No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGA20901953.219259132143757No Hit
TAGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA12138761.8695774309526791No Hit
ATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATGA10164281.565473614264035No Hit
TTTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAG9342271.438869962489273No Hit
TGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATGAT8349411.2859524776641609No Hit
ATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCC8203561.2634890737988198No Hit
TAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGAT8139151.253568828046563No Hit
AAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCC7580971.1675995255470355No Hit
ATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAG6955211.0712217428745923No Hit
AAATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGAT6430120.990348868444342No Hit
AGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAG6385310.9834473591731323No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGG5492400.8459238902296853No Hit
ACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCT5228800.805324964902953No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5022420.7735389018948686Illumina Single End Adapter 1 (95% over 22bp)
TTGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATGAT4754000.7321976138212666No Hit
ATCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAG3650410.5622258080499136No Hit
AAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGAT3644590.5613294281356436No Hit
AATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAG3415330.5260194523374393No Hit
AGAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT3330280.5129202922500394No Hit
AAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCA3008530.46336526863897665No Hit
AATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTAT2673720.41179878082166527No Hit
ATTTGATAAAGTGCTGATAGATTTATATGATTACAGTGCGTTAATTTTTC2667500.41084079404043505No Hit
TTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGG2619480.40344488966187025No Hit
AATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAG2512860.38702357927364484No Hit
TAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCC2503480.3855788982434296No Hit
ACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCAAATTAAC2480650.38206268631167956No Hit
AAAAATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAG2417850.3723904081989376No Hit
ATAGCCGTACTACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGGGC2402920.37009093188965037No Hit
AGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTT2310390.3558397233900959No Hit
ATATTTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT2274910.35037519429073144No Hit
AATTTGATAAAGTGCTGATAGATTTATATGATTACAGTGCGTTAATTTTT2227040.3430023924872767No Hit
AAGCAGAACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCA2196350.33827560561975994No Hit
TGAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGA2088210.321620189137086No Hit
AATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATG1908880.29400029050718113No Hit
AAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGA1898160.2923492264726494No Hit
AAAATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGA1897760.2922876196056893No Hit
AACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGAT1644430.25327045058815845No Hit
GGTTGATCACGAGTTAGTCGGTCCTAAGTTCAAGGCGAAAGCCGAAAATT1629740.2510079383990473No Hit
AAAAACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTT1592790.24531700406360432No Hit
TAAGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTA1503300.23153400775294697No Hit
AAGTCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA1472340.22676563625023213No Hit
ACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCTGC1357980.20915223298632804No Hit
AAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCG1328630.20463182912312775No Hit
TAGTATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGT1306400.20120802749181796No Hit
AAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCC1291760.1989532161610768No Hit
AGGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA1252190.19285875684704495No Hit
ATAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTT1231700.18970294508701177No Hit
ACTACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGGGCTAGAAGAG1224140.18853857530146512No Hit
TTATAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGA1210850.18649168714671446No Hit
AAGCAGCGTCGGGCGCGGTTAGTACTTAGATGGGGGACCGCTTGGGAACA1154200.17776661461348459No Hit
ACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATC1132270.1743890181323949No Hit
TTATAGCCGTACTACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGG1131690.1742996881753027No Hit
AAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCTGCC1106760.17046004019201197No Hit
TTACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGG1099770.16938346019188352No Hit
TAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTA1064380.16393279263758512No Hit
AATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTA1064290.1639189310925191No Hit
AGCAGAACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCAA970760.1495137054255643No Hit
CAGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA960360.14791192688460064No Hit
TATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAG954650.14703248885874465No Hit
GTTTGATCACGAGTTAGTCGGTCCTAAGTTCAAGGCGAAAGCCGAAAATT947680.1459589892019642No Hit
AGATACCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTG922960.14215168482382753No Hit
ATAAGTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT905940.13953031263467358No Hit
GACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTA897400.13821500602507458No Hit
AAATTTGATAAAGTGCTGATAGATTTATATGATTACAGTGCGTTAATTTT883910.13613731443684385No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATAAAGAAGCCGT813280.12525908190335708No Hit
AAACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCT813100.12523135881322503No Hit
AACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCAAATTAA808950.12459218756851358No Hit
GCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGT803950.12382210173151181No Hit
TGTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAG802710.12363112044393539No Hit
AGTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGA738920.11380636533546702No Hit
AATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGA738670.11376786104361693No Hit
AGAAAAGTTACCATAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG734950.11319491718088763No Hit
AACACGGACCAAGGAGTCTAACATATGTGCAAGTTATTGGGATATAAACC703330.10832489434768856No Hit
AAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCT681770.105004284218537No Hit
AACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGC677760.10438667537726158No Hit
AAAGGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGAT654160.1007518702266133No Hit
AAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCAC652170.10044537606348661No Hit
TGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTC649800.10008035537674778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)