FastQCFastQC Report
Fri 7 Jul 2023
SRR7132291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89912857
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG59297816.595031231184212No Hit
AAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA17481271.944245860188827RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC17087021.9003978485524047RNA PCR Primer, Index 1 (100% over 24bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA14564101.6198017153431128No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC14393601.6008389100571012RNA PCR Primer, Index 9 (100% over 50bp)
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG12645721.4064417950816535No Hit
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA12550751.3958793457091458RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT11543141.2838141713147877RNA PCR Primer, Index 1 (100% over 30bp)
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT11215791.2474066973536388RNA PCR Primer, Index 1 (100% over 30bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA9679491.0765412559407381RNA PCR Primer, Index 1 (100% over 28bp)
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA8974730.9981586949239084RNA PCR Primer, Index 1 (100% over 32bp)
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC7855390.8736670440802476No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG7561050.8409309026850298Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA6914560.7690290611052433RNA PCR Primer, Index 1 (100% over 22bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG6716130.7469599147539044No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG5200830.5784300681269643RNA PCR Primer, Index 6 (100% over 34bp)
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA5179600.5760688930171577No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC5096340.5668088157848216RNA PCR Primer, Index 1 (100% over 26bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT4973010.5530922012632743RNA PCR Primer, Index 1 (100% over 25bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC4778510.531460144793308No Hit
AGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAACT4764240.5298730525268482RNA PCR Primer, Index 1 (100% over 25bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG4694320.5220966340775935No Hit
AAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGAACTCCAG4561850.5073634797301569RNA PCR Primer, Index 1 (100% over 29bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG4158200.4624700113800188RNA PCR Primer, Index 1 (100% over 29bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC3968180.4413362151310574RNA PCR Primer, Index 1 (100% over 26bp)
TGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAA3958930.4403074412372416RNA PCR Primer, Index 1 (100% over 23bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC3891310.4327868260264492No Hit
ATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3787890.42128457779959094RNA PCR Primer, Index 1 (100% over 31bp)
AAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGAACTC3724990.414288915321643RNA PCR Primer, Index 1 (100% over 26bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC3625040.40317259632846497RNA PCR Primer, Index 1 (100% over 27bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA3620280.4026431948436473No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC3409540.37920494507253844No Hit
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT3369430.3747439590313541RNA PCR Primer, Index 1 (100% over 30bp)
ATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAAGGAAC3205110.3564684859252109RNA PCR Primer, Index 1 (100% over 24bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC3202880.35622046800270174No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA3070990.34155182055887734No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG3045650.3387335361838185Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAACTCCAG2874060.3196495024065357RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2847710.3167188870441521No Hit
AAGGATTGGCTCTGAAGATTGAGTGGAATTCTCGGGTGCCAAGGAACTCC2785210.30976771208593673RNA PCR Primer, Index 1 (100% over 27bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG2562280.2849737051509775Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC2423850.26957768675952537No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA2379340.26462733800128274No Hit
ACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT2359290.26239740107468723RNA PCR Primer, Index 1 (100% over 30bp)
AAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2257110.2510330641590001RNA PCR Primer, Index 1 (100% over 33bp)
ATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2243500.2495193763000991RNA PCR Primer, Index 1 (100% over 32bp)
ATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG2240320.2491657005182251RNA PCR Primer, Index 1 (100% over 29bp)
ATGGGACTACCCCCTGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2212120.24602933037707833RNA PCR Primer, Index 1 (100% over 29bp)
AAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAGG2191510.24373711092285721Illumina Small RNA Adapter 2 (100% over 21bp)
GATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAAC2172370.24160838310365337RNA PCR Primer, Index 1 (100% over 24bp)
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA2165890.2408876852839856RNA PCR Primer, Index 1 (100% over 23bp)
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA2144690.2385298467381589No Hit
ATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2121270.23592510245781648RNA PCR Primer, Index 1 (100% over 31bp)
AACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCC2099170.2334671669925915RNA PCR Primer, Index 1 (100% over 27bp)
AGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCC2090630.23251735844630095No Hit
ATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAA2083970.23177664124275354No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG1975040.2196615774315791No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC1934800.2151861329464817No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA1911900.21263922244179162RNA PCR Primer, Index 1 (100% over 22bp)
AACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCAAGGAACTCCA1910160.2124457017309549RNA PCR Primer, Index 1 (100% over 28bp)
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCC1886060.20976532866706704No Hit
AAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGGAACTC1870830.2080714663532491RNA PCR Primer, Index 1 (100% over 26bp)
AAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1834910.2040764870812636RNA PCR Primer, Index 1 (100% over 31bp)
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG1834470.20402755080955776Illumina Small RNA Adapter 2 (100% over 21bp)
AGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGA1799880.20018049254068304RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1784270.19844436708311916No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1775670.19748788540886872Illumina Small RNA Adapter 2 (100% over 21bp)
ACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1773960.19729770126201196RNA PCR Primer, Index 1 (100% over 31bp)
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA1725650.19192472106630978RNA PCR Primer, Index 1 (100% over 22bp)
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG1715400.19078472837316246No Hit
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA1695750.18859927896629955No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGGA1686670.18758941226837003RNA PCR Primer, Index 1 (100% over 22bp)
AACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1666310.1853249975139818RNA PCR Primer, Index 1 (100% over 29bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA1662220.18487011262471617No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1651210.18364559364407695No Hit
TAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1635250.18187054160674707RNA PCR Primer, Index 1 (100% over 32bp)
AGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1634770.181817156583068RNA PCR Primer, Index 1 (100% over 28bp)
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC1617260.17986971540677435No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA1597190.17763755410419224No Hit
GATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGG1579040.1756189328963265Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAA1575000.17516960894702746RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGTGCCAAGGAACTCC1568070.17439886266766053RNA PCR Primer, Index 1 (100% over 27bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA1539950.17127139003046027No Hit
AGATAACATGCAGATCGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG1521280.1691949350469422RNA PCR Primer, Index 1 (100% over 29bp)
AATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCAAGGAAC1518540.168890195536774RNA PCR Primer, Index 1 (100% over 24bp)
GATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAAC1513610.16834188685607RNA PCR Primer, Index 1 (100% over 24bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1505540.16744435114546521No Hit
ACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTG1473890.16392427614662494No Hit
AATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAA1469560.16344269874551978RNA PCR Primer, Index 1 (100% over 23bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA1454800.16180110926738767RNA PCR Primer, Index 1 (100% over 23bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC1412950.15714660251536663No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1402590.15599437575429284No Hit
AATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAAGGAACT1401560.15587982039098144RNA PCR Primer, Index 1 (100% over 25bp)
TTGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTG1384170.15394572547060761No Hit
AGATAACATGCAGATCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1378810.15334959270619106RNA PCR Primer, Index 1 (100% over 31bp)
TGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAA1360370.1512987180465192RNA PCR Primer, Index 1 (100% over 23bp)
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG1360340.15129538148253927No Hit
AAATTAGAGTGCTTAAAGCAGGCTTCTGGAATTCTCGGGTGCCAAGGAAC1338130.14882521194938783RNA PCR Primer, Index 1 (100% over 24bp)
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG1330480.14797438813450228No Hit
AAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1326970.1475840101488489RNA PCR Primer, Index 1 (100% over 32bp)
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGGAATTCTCGGGTGCC1308990.14558429613686952No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG1290500.14352786053723104No Hit
ACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCCAAGGAACTCC1265990.14080188776561733RNA PCR Primer, Index 1 (100% over 27bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT1226940.13645879365172436No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1211570.13474936070600002Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT1209010.13446464057971153RNA PCR Primer, Index 1 (100% over 25bp)
TAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAAGG1166370.12972227097621866Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGTATGGCTTCTAAACCATTTGGAATTCTCGGGTGCCAAGGAACTCCA1129030.12556936100918248RNA PCR Primer, Index 1 (100% over 28bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG1122760.12487201913737431No Hit
ATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGAACT1119070.12446162176784129RNA PCR Primer, Index 1 (100% over 25bp)
TAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGGAATTCTCGGGTGCCAAG1104270.12281558353773588No Hit
AACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACT1078520.11995169945495114RNA PCR Primer, Index 1 (100% over 25bp)
TTAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG1077920.11988496817535227Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAACT1059780.11786745915547985RNA PCR Primer, Index 1 (100% over 25bp)
AGATGGGCTAGAAGAGCATGACATTGGAATTCTCGGGTGCCAAGGAACTC1058180.11768950907654953RNA PCR Primer, Index 1 (100% over 26bp)
AACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAAC1044080.11612132400597615RNA PCR Primer, Index 1 (100% over 24bp)
ACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1040530.1157264972683495RNA PCR Primer, Index 1 (100% over 32bp)
AAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGAA1039990.11566643911671051RNA PCR Primer, Index 1 (100% over 23bp)
TAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1034590.1150658576003207RNA PCR Primer, Index 1 (100% over 31bp)
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC1022620.11373456857232331RNA PCR Primer, Index 1 (100% over 24bp)
AAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1000800.11130777437091115RNA PCR Primer, Index 1 (100% over 31bp)
ATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC994160.11056928154335036RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC990130.11012106978204463No Hit
ACTGAAGCTTATCCTTTGCTTGATGATTCGATGGAATTCTCGGGTGCCAA985610.10961836080906649No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA983210.10935143569067102No Hit
ACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGAACT949050.10555220150550881RNA PCR Primer, Index 1 (100% over 25bp)
AAAACGACAATGGATGTGATGCCAATGTGGAATTCTCGGGTGCCAAGGAA945680.10517739415176186RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC937710.10429098032109024No Hit
AACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGCCAAG915770.1018508398637583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)