FastQCFastQC Report
Mon 10 Jul 2023
SRR7132292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69602339
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1377420019.789852177237893No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC952256513.681386483290453RNA PCR Primer, Index 9 (100% over 50bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA14314332.0565874948541603No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC13578741.9509028281362786No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC12896891.8529391663116381No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC5990790.8607167641305847No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA5837160.8386442300452001No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC5722130.8221174865976846No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG5711640.820610353338844No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG5429320.7800484980828015No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA4655850.6689214855265138No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC4169270.5990129153561923No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3998150.574427534683856No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC3974930.5710914399011792No Hit
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA3915200.5625098317457407No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC3656600.5253559079386685No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC3631010.5216793073577599No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA3192420.45866562041830233No Hit
TGTTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG3121160.44842745873813233No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC3074860.44177538343934103No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG2961160.42543972552416665No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA2827920.40629669069023666No Hit
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG2596370.37302913052965075No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA2573110.36968728881367047No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG2505370.3599548572642078No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATG2268750.32595887330740425RNA PCR Primer, Index 9 (100% over 49bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC2139670.3074135195370374No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC2033790.2922013870826956No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1989980.2859070583820466No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA1978760.2842950435904173No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC1973000.2834674851947145RNA PCR Primer, Index 9 (100% over 50bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1858820.26706286407989827RNA PCR Primer, Index 1 (100% over 22bp)
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA1847020.26536751875536824No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA1782900.2561551846698715No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1754490.25207342529106674RNA PCR Primer, Index 1 (100% over 27bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG1665380.23927069462421371Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT1644430.23626073830651007RNA PCR Primer, Index 1 (100% over 25bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC1606720.23084281693464354RNA PCR Primer, Index 1 (100% over 26bp)
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA1588400.22821072148164448RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG1504160.21610767994449154No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1389080.1995737528303467No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC1319470.18957265214894575No Hit
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1313310.18868762442020806RNA PCR Primer, Index 9 (98% over 50bp)
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA1308700.18802529035698068RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG1301020.18692187916271033No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC1265290.18178843098936662No Hit
TGCATGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1234630.17738340661224045No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG1200090.1724209297046756No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC1197290.1720186443734312No Hit
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA1155050.16594988280494424No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC1150580.16530766300827907No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC1150410.16528323854173924No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC1142340.16412379474775984RNA PCR Primer, Index 9 (98% over 50bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC1123230.1613781973620168RNA PCR Primer, Index 1 (100% over 27bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGC1116330.16038685136716455No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG1106940.15903775877416992No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC1053930.15142163541371792RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG1037550.1490682662259382Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTG1035440.148765115494179No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTG993070.1426776763924557No Hit
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA992930.1426575621258935RNA PCR Primer, Index 1 (100% over 22bp)
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA990180.14226246046127847No Hit
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG983970.14137024906591142Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG974220.13996943407318538Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA948770.13631294775883895No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCC938280.13480581449999834No Hit
TGATTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG896950.1288677956641658No Hit
TTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGT896610.12881894673108615No Hit
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC894760.12855315106579968RNA PCR Primer, Index 1 (100% over 24bp)
TGCTTGGACTACATATGATTGAGGGTTGTATGGAATTCTCGGGTGCCAAG869560.12493258308460006No Hit
TGCCTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG865200.1243061673545195No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG864560.12421421642166365No Hit
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA861520.1237774494905983RNA PCR Primer, Index 1 (100% over 32bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCA849150.12200021036649358No Hit
TGCTTGGGCTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG809880.11635815859579086No Hit
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT798760.11476051113742025RNA PCR Primer, Index 1 (100% over 30bp)
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC796000.11436397273947933RNA PCR Primer, Index 1 (100% over 24bp)
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC787100.1130852800794525No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG769990.11062702935888405Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCA761890.10946327536492703No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA748810.10758402817468533RNA PCR Primer, Index 1 (100% over 22bp)
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG738220.106062527582586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG735580.10568322998455555No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC734300.10549932811884381No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGATGGAATTCTCGGGTGCCAA732170.10519330392043291No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT712890.10242328206815003No Hit
ACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA701090.10072793674362006RNA PCR Primer, Index 9 (100% over 35bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA700660.10066615721060752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)