FastQCFastQC Report
Fri 7 Jul 2023
SRR7132293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57310413
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT28373124.950779189115249No Hit
TAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGT21572063.7640733805216167No Hit
TTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGA18438593.2173193377615337No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGA16482452.8759956763878143No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA14216632.4806364595557877Illumina Single End Adapter 1 (95% over 22bp)
AAGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA14147162.4685147531566383No Hit
AAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCC8779761.5319659273786772No Hit
TAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGAT7376001.2870261465398967No Hit
TTTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAG6422251.1206078727787216No Hit
AAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCAC6214701.084392813571244No Hit
ATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATGA5603600.9777629765117902No Hit
AAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGAT5125500.8943400913896747No Hit
TAGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA4859060.8478494126364087No Hit
TGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATGAT4755920.8298526831415435No Hit
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTAGGCACCATC4612300.804792664816427No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGG4421410.7714845816937316No Hit
AAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACC4257780.7429330512763885No Hit
ATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCC4242250.7402232470388933No Hit
ATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAG3799390.6629493317383701No Hit
AAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCA3417740.5963558489798354No Hit
AATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTAT3240190.5653754405852912No Hit
AAATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGAT3126540.5455448384222951No Hit
AATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAG3002710.5239379447501102No Hit
ATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGACACGCCTGTAGGCA2860770.499171066870518No Hit
GAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTATCCGCTGTAGG2807690.48990922469883436No Hit
TTGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATGAT2724560.47540400729619586No Hit
TAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCC2580570.4502794282777198No Hit
AATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAG2491330.4347080869928472No Hit
AGCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAG2298900.4011312917950879No Hit
ACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCAAATTAAC2146200.37448691915725685No Hit
AGAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT2071560.3614631079346785No Hit
TTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGG2061030.35962574549933884No Hit
ATTATATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACCATCAATTCG1675660.2923831660400005No Hit
TGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTC1630580.2845172307517658No Hit
AAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATC1630300.2844683740108451No Hit
AAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCG1602830.2796751787498024No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1543990.26940828362203567Illumina Single End Adapter 1 (95% over 22bp)
GCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCA1511150.26367808586547786No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1506890.26293476545004135Illumina Single End Adapter 1 (95% over 22bp)
ATATTTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT1502880.26223506712471256No Hit
GACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTA1484500.2590279710599887No Hit
AAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCTGCC1463110.25529566503036716No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1410610.2461350261077337Illumina Single End Adapter 1 (95% over 22bp)
AGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTAGGCACCATCAAT1361290.23752926017127116No Hit
CTGGAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1333450.2326715042168689Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1311920.22891476981678704Illumina Single End Adapter 1 (95% over 22bp)
TGAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGA1310840.2287263223875214No Hit
ATCCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAG1305220.22774569780189857No Hit
ACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCTGC1297750.22644226974947815No Hit
AAGCAGAACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCA1293910.22577223444542266No Hit
ATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGACACGCTGTAGGCAC1291510.22535346238038803No Hit
AGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTT1279520.22326134693881894No Hit
AAAAATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAG1228970.21444096031902615No Hit
AAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCC1196550.20878404767384942No Hit
ACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCT1180560.20599397879055592No Hit
AAAAACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTT1174460.20492959979192613No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGCTGT1140490.19900223018808116No Hit
AAGTTGTATACCTATACATTACCGCTAAAGTAGATGATTTATATCTGTAG1122600.19588063341996853No Hit
ATAGCCGTACTACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGGGC1086090.18951006338062856No Hit
ATTTGATAAAGTGCTGATAGATTTATATGATTACAGTGCGTTAATTTTTC1077960.1880914730103236No Hit
GAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTCTG1043110.1820105536492993No Hit
TTACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGG1034100.18043841352181497No Hit
AAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCT983240.1715639355102885No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA957890.16714065557335978Illumina Single End Adapter 1 (95% over 22bp)
TTGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTC950650.1658773598438385No Hit
TAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTA924430.1613022750333347No Hit
ATATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACCATCAATTCGTAT917920.16016635580692815No Hit
TTATAGCCGTACTACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGG905810.15805330176210736No Hit
AATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGATG905640.15802363874083408No Hit
AAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTAGGCACCATCAATTCGT892650.1557570349388339No Hit
ATATGATTACAGTGCGTTAATTTTTCGGAATTATATAATGGCACTGTAGG892650.1557570349388339No Hit
AAAATGGATGGCGCTTAAGTTGTATACCTATACATTACCGCTAAAGTAGA892100.15566106634059676No Hit
GAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTATCCGTCTGTAG876420.1529250888490369No Hit
ATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTATC866310.15116101152507835No Hit
AATTTGATAAAGTGCTGATAGATTTATATGATTACAGTGCGTTAATTTTT865380.15099873734987743No Hit
ATGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTAGGCACCATCAAT857930.1496987990646656No Hit
ATGATTACAGTGCGTTAATTTTTCGGAATTATATAATGGCACTGTAGGCA837090.1460624616332812No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG805770.1405974861845787Illumina Single End Adapter 1 (95% over 22bp)
TTATAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGA764190.133342260157853No Hit
ATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACCATCAATTCGTATGC756560.1320109139677636No Hit
TAGTATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGT754700.13168636561736172No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA746410.1302398571093878Illumina Single End Adapter 1 (95% over 22bp)
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGCTGTAG722250.12602421832137206No Hit
AGTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGA716610.1250401039685406No Hit
TATAATTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAG706410.12326032269214322No Hit
CGGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA704300.12289215225163358Illumina Single End Adapter 1 (95% over 22bp)
AACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCTGTAGGCACCATCA687780.12000960453731158No Hit
ATAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTT681070.11883878763881879No Hit
ACTACCATGGAAGTCTTTCGCAGAGAGATATGGTAGATGGGCTAGAAGAG657450.11471737256543589No Hit
AACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCAAATTAA655970.11445912979199784No Hit
ATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCTGCCCCTGTA653250.1139845214516252No Hit
ATTTTCTCCTCGGACCTTGAAAATTTATGGTGGGGACACGCACTGTAGGC641530.11193951786737255No Hit
AAGTTGTATACCTATACATTACCGCTAAAGTAGATGATTTATATTCTGTA633740.11058025353961416No Hit
AAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCT630980.11009866566482429No Hit
AAGCAGCGTCGGGCGCGGTTAGTACTTAGATGGGGGACCGCTTGGGAACA629840.10989974893393281No Hit
AAACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTCT614120.10715679190795571No Hit
AGCAGAACTGGCGCTGTGGGATGAACCAAACGTAATGTTACGGTGCCCAA606310.10579403781298871No Hit
AAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGT602480.10512574739253754No Hit
ATAAGTTTGAACGAAAGGGAATACGGTTCCAATTCCGTAACCTGTTGAGT596990.10416780629377072No Hit
AAAACAAAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGTGATTTC591030.1031278556656013No Hit
ATGGCTCTCTCAGTCGCTTCCCGGGAAACCAAAGCTTTTGGGCTCCGGGG583890.10188200877212314No Hit
AAGTCTAGAATCAATTGTAAACGACTTTTGTAACAGGCAAGGTGTTGTAA582620.10166040855437562No Hit
ACTTTTGTAACAGGCAAGGTGTTGTAAGTGGTTGAGCAGCTGCCCTGTAG578560.1009519858110253No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)