FastQCFastQC Report
Fri 7 Jul 2023
SRR7132294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106961271
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1888461117.655559646444367No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA32716883.058759464442041No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC25121572.3486603856829635No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG19129931.7884912755010176No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC15321831.4324652144419638No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG14935411.3963381194301627No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC13707061.2814974870670712RNA PCR Primer, Index 10 (100% over 50bp)
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC12964311.2120564648114551No Hit
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA11330781.0593348315765618RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC9720000.9087401364181621RNA PCR Primer, Index 1 (100% over 24bp)
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG9202290.8603385051398651No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC9140390.8545513637361322No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA8427170.7878711538496957No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC8376560.7831395346826049No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC7749090.7244762452383349RNA PCR Primer, Index 1 (100% over 26bp)
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA7682560.7182562368766168No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG7152560.6687055915780956Illumina Small RNA Adapter 2 (100% over 21bp)
AAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA6475890.6054425063815855RNA PCR Primer, Index 1 (100% over 22bp)
TGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAA6055580.5661469748241865RNA PCR Primer, Index 1 (100% over 23bp)
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT5978230.55891538536411RNA PCR Primer, Index 1 (100% over 25bp)
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA5810890.5432704702994787No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG5591830.5227901601879805No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC5484660.5127706457414852No Hit
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5311640.4965946973461076RNA PCR Primer, Index 1 (100% over 32bp)
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA4581950.42837467778407384No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC4557290.42606917040093883No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG4388630.41030084618197926No Hit
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA4311770.40311506769585786No Hit
ACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT4303710.402361523920186RNA PCR Primer, Index 1 (100% over 30bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA4285040.4006160323207079RNA PCR Primer, Index 1 (100% over 22bp)
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA4194130.39211669427525786RNA PCR Primer, Index 1 (100% over 22bp)
AGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAACT4088000.3821944112836879RNA PCR Primer, Index 1 (100% over 25bp)
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG3814560.35663001798099425Illumina Small RNA Adapter 2 (100% over 21bp)
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG3773540.3527949850184559No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC3706940.346568432231887No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA3555390.3323997524300174No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC3554510.33231747966046515No Hit
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG3457280.32322727354277603Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC3417380.31949695137784967No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGGA3390870.31701848419508777RNA PCR Primer, Index 1 (100% over 22bp)
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA3244040.30329108561172574No Hit
ATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAA3239100.30282923620083013No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGG3146280.2941513288487382No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCC3123750.29204495896463306No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC2992550.27977883695865957RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2987620.2793179224655997No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC2831610.2647322693089539No Hit
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC2822070.2638403576935805RNA PCR Primer, Index 1 (100% over 27bp)
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG2821190.26375808492402825No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA2810560.2627642672645503No Hit
ATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAAGGAAC2628750.24576652609148594RNA PCR Primer, Index 1 (100% over 24bp)
AGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTCC2495920.23334801247827355RNA PCR Primer, Index 1 (100% over 27bp)
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG2414330.22572001785580875Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGT2334310.21823880533356788No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA2323650.21724218292058253No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2322450.217129992780284No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA2316940.21661485305274653No Hit
ATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2299030.21494041520879084RNA PCR Primer, Index 3 (100% over 34bp)
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG2189390.20468997605684772Illumina Small RNA Adapter 2 (100% over 21bp)
TTGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT2183620.20415052846557893RNA PCR Primer, Index 1 (100% over 25bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG2181900.2039897225978177No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG2175200.2033633276478175No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG2074790.1939758176583373No Hit
TAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGAACTC2071960.1937112359107999RNA PCR Primer, Index 1 (100% over 26bp)
ATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCA2005340.18748281328855937RNA PCR Primer, Index 1 (100% over 28bp)
TTGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTG2000610.18704059715221596No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG1992910.18632071041863368Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA1963580.18357859640617025No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGGA1950730.18237722698714004RNA PCR Primer, Index 1 (100% over 22bp)
GATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGG1948940.18220987669452807Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1899030.1775437017759447No Hit
TTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTG1855940.17351514082139133No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC1796320.1679411606842256No Hit
TGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAAC1753440.1639322330042245RNA PCR Primer, Index 1 (100% over 24bp)
TTAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG1723380.1611218699897461Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC1671240.1562472083937746No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA1651650.15441570435340096No Hit
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA1648530.15412400998862477RNA PCR Primer, Index 1 (100% over 22bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG1599070.14949990637265334Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGTGGAATTCTCGGGTGCCAAGG1594320.14905582040063828Illumina Small RNA Adapter 2 (100% over 21bp)
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA1579520.14767214200362297RNA PCR Primer, Index 1 (100% over 22bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC1548440.14476641736989082No Hit
ATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1519970.142104706291308RNA PCR Primer, Index 1 (100% over 30bp)
ACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCC1475430.13794058225056058RNA PCR Primer, Index 1 (100% over 27bp)
TCGACATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGC1464880.13695424393376926No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCA1460400.13653540074332138No Hit
TAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAA1452510.1357977505708585RNA PCR Primer, Index 1 (100% over 23bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCA1440430.13466836982518654No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGC1427810.13348850351638025No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTTGGAATTCTCGGGT1411310.13194588908727534No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAG1395850.13050050611309583No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG1395800.13049583152391672No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAA1368780.12796968353152796No Hit
GATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAAC1368400.12793415665376678RNA PCR Primer, Index 1 (100% over 24bp)
ACTTTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGC1366970.12780046340324433No Hit
AGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1341100.125381830961975RNA PCR Primer, Index 1 (100% over 28bp)
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC1332520.12457967145884047No Hit
TACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGC1325780.123949536837497No Hit
ATGGATGTGATGCCAATGTAATTTGTAACTGGAATTCTCGGGTGCCAAGG1324060.12378873096973576Illumina Small RNA Adapter 2 (100% over 21bp)
TTTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCA1311710.12263410744249663No Hit
AACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTGCCAAGGAA1254040.11724243628331604RNA PCR Primer, Index 1 (100% over 23bp)
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG1251950.11704703845562942No Hit
TTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAA1199640.11215648325644896RNA PCR Primer, Index 1 (100% over 23bp)
TTGCATTGTATGGCTTCTAAACCATTTGGAATTCTCGGGTGCCAAGGAAC1193830.11161329599383688RNA PCR Primer, Index 1 (100% over 24bp)
AGTTACATATAAAAGCATTATTTAATACAATGTGGAATTCTCGGGTGCCA1193780.11160862140465776No Hit
ATGTGATGCCAATGTAATTTGTAACATAGTTGGAATTCTCGGGTGCCAAG1174480.10980422998152294No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC1163380.10876647118376145No Hit
ATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1158230.10828498849831357RNA PCR Primer, Index 1 (100% over 30bp)
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGT1157770.1082419822778658No Hit
AAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGGAACTC1153740.10786521039002986RNA PCR Primer, Index 1 (100% over 26bp)
TGGGTTTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGC1144840.10703313351614904No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG1141050.10667879965637282No Hit
ATGTGATGCCAATGTAATTTGTAACATTGGAATTCTCGGGTGCCAAGGAA1137440.10634129431764139RNA PCR Primer, Index 1 (100% over 23bp)
TTATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCC1123970.10508195999279028No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC1115390.10427980048965572No Hit
TAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAACTCC1112130.10397501727517804RNA PCR Primer, Index 1 (100% over 27bp)
ATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG1085140.10145167403629674RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)