FastQCFastQC Report
Fri 7 Jul 2023
SRR7132295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7132295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43401129
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG790368218.210775116011384No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCA8804372.028603910280767No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCC8794102.0262376123902213No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATGGAATTCTCGGGTGCC7412051.707801195678573No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGC6689021.5412087551915987No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC6236131.4368589351673315RNA PCR Primer, Index 8 (100% over 50bp)
ATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAG5006621.1535690695972447No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC4316510.9945616852501693No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTGGAATTCTCGGGTGC4064990.9366092757633102No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTGGAATTCTCGGGTG3788010.872790659431924No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA3322350.7654985196352841No Hit
AGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGGA3290900.7582521643618995RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCC3246760.7480819220163605No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3194110.7359509011850821No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCC3181520.7330500549882009No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCAA3155010.7269419189533065No Hit
TTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTG2857090.6582985433397366No Hit
TTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2674680.6162696827541053No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGC2652930.6112582923822097No Hit
TGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAG2645200.6094772327236003No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAA2429020.5596674685582488No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCCA2425670.5588955992366005No Hit
TCTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCA2031080.46797860949654096No Hit
TAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA1987330.457898226564567RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1907700.43955077758461075No Hit
TGTTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1771870.4082543567011817No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGCC1756520.40471758234676336No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGTGGAATTCTCGGGTGCC1692840.3900451529728639No Hit
ATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCAA1575080.362912218251281No Hit
TACTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1501960.34606473025160245No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAAG1335020.3076002930707171No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGGA1333750.30730767395474895RNA PCR Primer, Index 1 (100% over 22bp)
TAAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCC1319680.30406582280382616No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAATGGAATTCTCGGGTGCCA1307510.3012617482830919No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGC1235370.28464006086108956No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1201780.2769006308568609No Hit
ATGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACT1200910.2767001752419851RNA PCR Primer, Index 1 (100% over 25bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGC1180240.27193762632303875No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCATGGAATTCTCGGGTGC1148000.26450924813499666No Hit
TTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGA1090180.25118701405209987RNA PCR Primer, Index 1 (100% over 22bp)
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTG1077530.24827234333005482No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATTGGAATTCTCGGGTGC1051120.24218724817043355No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTTGGAATTCTCGGGTGCCAA1048210.24151675869998682No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGC1016380.2341828481005644No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTGGAATTCTCGGGTGC1012880.23337641746600646No Hit
TTTGCATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGT1011780.23312296783800254No Hit
TATAGCTAATTAACACAATCCCGGGGCGTTCTATGGAATTCTCGGGTGCC1008170.23229119224064423No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACTGGAATTCTCGGGTGC985340.22703096041579932No Hit
TGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGGAA954210.2198583359432885RNA PCR Primer, Index 1 (100% over 23bp)
GCGCAGTGGAAGCGTGCTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC931750.21468335535695396RNA PCR Primer, Index 1 (100% over 27bp)
TGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGG919070.21176177237232702Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCAGTGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCCAAGGAACTC918930.21172951514694469RNA PCR Primer, Index 1 (100% over 26bp)
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCC901310.20766971292382738No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGGAATTCTCGGGTGCCA899030.20714438096760107No Hit
ATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGG872100.20093947325655975Illumina Small RNA Adapter 2 (100% over 21bp)
AGGACATTGTAATCTATTAGCATATACCATGGAATTCTCGGGTGCCAAGG871100.20070906450382892Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGG843910.19444425051707756Illumina Small RNA Adapter 2 (100% over 21bp)
TTATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAA818610.18861490907298747No Hit
TGCATTGTATGGCTTCTAAACCATTTAAAGTTTATTGGAATTCTCGGGTG814240.18760802282355374No Hit
GATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAA810180.18667256328746656No Hit
AAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTGCCAAGGAAC791610.18239387274925498RNA PCR Primer, Index 1 (100% over 24bp)
TAAACGACAATGGATGTGATGCCAATGTGGAATTCTCGGGTGCCAAGGAA766160.1765299699922553RNA PCR Primer, Index 1 (100% over 23bp)
TACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGGAATTCTCGGGTGC750650.17295633023740006No Hit
TTATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCC719680.16582057116532614No Hit
TGCATGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG713750.1644542472616323No Hit
TGTATGTAAGCGTATTACCGGTGGTGGAATTCTCGGGTGCCAAGGAACTC687540.15841523385255715RNA PCR Primer, Index 1 (100% over 26bp)
TATAGCTAATTAACACAATCCCGGGGCGTTCTTGGAATTCTCGGGTGCCA665610.15336236990517No Hit
TTTGATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCA664480.15310200801458412No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAA651250.1500537002159552No Hit
TATAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAG634680.1462358271832053No Hit
TTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGCCA616430.1420308674458676No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTGGAATTCTCGGGTGCCAAG614910.14168064614171674No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAGG587890.1354550016429296Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG587850.1354457852928204No Hit
AGGACATTGTAATCTATTAGCATATACCAAATTGGAATTCTCGGGTGCCA585480.1348997165488483No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGGAATTCTCGGGTGCCAA580530.1337591932228307No Hit
AAGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAGG568350.13095281461456915Illumina Small RNA Adapter 2 (100% over 21bp)
TGATTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG554190.12769022667590052No Hit
TTTTGATGCAAGCTTCTTGATCAAAGTATCACGAGTGGAATTCTCGGGTG546230.1258561730041631No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCA544900.12554972936303108No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGATGGAATTCTCGGGTGCCAA539490.12430321801075728No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTTGGAATTCTCGGGTGCCAAGG538210.1240082948072618Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGA535940.12348526693856281RNA PCR Primer, Index 1 (100% over 22bp)
TCGACATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCA532980.12280325703047956No Hit
TTTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG531590.1224829888641837No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATATGGAATTCTCGGGTGCCAA531150.12238160901298213No Hit
TATTGGTTGAACTCTAGATAACATGCAGATCGTATGGAATTCTCGGGTGC527000.12142541268914918No Hit
ATTGGTTGAACTCTAGATAACATGCAGATCGTATGTGGAATTCTCGGGTG523480.12061437387953662No Hit
TGCTTGGACTACATATGATTGAGGGTTGTATGGAATTCTCGGGTGCCAAG521190.12008673783578303No Hit
TGCCTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG511430.11783794840913009No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG509690.11743703717937845No Hit
TAAGGACATTGTAATCTATTAGCATATACCTGGAATTCTCGGGTGCCAAG503800.11607992962579385No Hit
TAGCTAATTAACACAATCCCGGGGCGTTCTATTGGAATTCTCGGGTGCCA494890.11402698763896212No Hit
ATGCAAGCTTCTTGATCAAAGTATCACGTGGAATTCTCGGGTGCCAAGGA494800.11400625085121634RNA PCR Primer, Index 1 (100% over 22bp)
TTTGATGCAAGCTTCTTGATCAAAGTATCTGGAATTCTCGGGTGCCAAGG482870.1112574744311375Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA476090.10969530308762244No Hit
TGCTTGGACTACATATGGTTGATGGTTGTATGGAATTCTCGGGTGCCAAG475800.1096284845493305No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG474320.10928747959528887Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTGGAATTCTCGGGTGCCAA471290.10858934107451444No Hit
ACCCGGAAGGCCCGGGTTCAATTCCCGGTATGGGTGGAATTCTCGGGTGC460470.10609631836996684No Hit
ATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA460420.10608479793233028RNA PCR Primer, Index 1 (100% over 32bp)
AGTTACATATAAAAGCATTATTTAATACAATGTGGAATTCTCGGGTGCCA459470.10586590961723599No Hit
ATTGTATGGCTTCTAAACCATTTAAAGTTTATGGAATTCTCGGGTGCCAA458480.10563780495203247No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG454830.10479681300456492No Hit
AAAACGACAATGGATGTGATGCCAATGTTGGAATTCTCGGGTGCCAAGGA448250.10328072341159605RNA PCR Primer, Index 1 (100% over 22bp)
TGCTTGGGCTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG447780.10317243129781256No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGTGGAATTCTCGGGTGCCAAG447760.10316782312275792No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTGGAATTCTCGGGTGCCAAG440530.101501967840514No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAAATTGGAATTCTCGGGTGCC434440.10009877853638324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)