FastQCFastQC Report
Fri 7 Jul 2023
SRR7182546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7182546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21503567
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAA5468102.5428804439747137No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA3732641.735823642654263No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAAA1904020.8854437963710857No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAA1399640.6508873620827651No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA922520.4290078943646884No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAAA891180.41443356816104043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870220.4046863480835528No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAAA862790.40123110737860374No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAAA806030.37483548659624705No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAA797040.37065478485499637No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGATAAAAAAAAAA773400.35966125992027276No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAA609850.28360411089006765No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGATAAAAAAAAA561700.2612124769811446No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAAAAAAAAAAAAAAAAA540650.2514234033823319No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAA492830.2291852323849341No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA471660.2193403540910213No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAAAAAAAAA417460.19413523347080044No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAA406960.18925232264953995No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAA386090.17954695609337742No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA384290.17870988566687562No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGAAAAAAAAAAAAA371110.17258066998837915No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA366280.1703345310105993No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAA344500.16020597884992754No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAAAAAAAAAAA331220.1540302592588476No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCAAAAAAAAAAAAAA330280.15359312248056334No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAAAAAAAA307710.14309718941048244No Hit
TCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAAAAAAAAAAAAAAAAA301710.14030695465547646No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGAAAAAAAAA300970.13996282570235904No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAA298600.13886068297413168No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAA294380.13689821786311082No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGAAAAAAAAAAAA277440.1290204550714772No Hit
TCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCAAAAAAAAAAAAAAAAAA269770.12545360497632788No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAAAAAAAAAAA252800.11756189101091927No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCAAAAAAAAAAAAA250230.11636674045752501No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGAAAAAAAA250010.11626443184984148No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAAAAAAA241310.11221859145508278No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGGAAAAAAAAAA238940.1111164487268554No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA230900.10737753415514739No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAAAAAAAAA215510.10022058200855699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)