FastQCFastQC Report
Fri 7 Jul 2023
SRR7182548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7182548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22603759
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAA6387112.8256848783425803No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA3505711.5509411509828963No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAAA1839570.8138336636839917No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAAA1217060.5384325677866234No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAA1183690.5236695365580566No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAA1015350.4491951980199399No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAAA968060.4282738990448447No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAAAAAAAAAAAAAAAAA887380.3925807207553399No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA732140.3239018784442004No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGATAAAAAAAAAA724070.3203316758066656No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAAA665790.29454835366099946No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAAAAAAAAA552720.24452570034922066No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAA543870.24061042236382008No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAA511270.22618804244019766No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGATAAAAAAAAA490500.21699930529254005No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAA433770.1919017097996842No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG431480.19088860397069354No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAA429080.18982683366956796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA425060.18804836841518263No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA412510.1824961945488801No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG411990.18226614431696958No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGAAAAAAAAA380400.16829059272840416No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA350000.15484150224748017No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA349110.15444776242747943No Hit
TCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAAAAAAAAAAAAAAAAA339620.15024934569511203No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAA339350.15012989653623543No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAAAAAAAA335850.1485814815137606No Hit
TCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCAAAAAAAAAAAAAAAAAA302900.1340042600878907No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAAAAAAAAAAAAAAAAAAA299930.13269031934024778No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAAAAAAAAAAA291970.12916878117484795No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAAAAAAAAAAA273690.12108163071460813No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTAAAAAAAAAAAAAAAAAAA269960.11943146270494212No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGAAAAAAAA267000.11812194600022059No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAAAAAAAAAAAAAAAAAAAGA265610.11750700403415201No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAAAAAAA260700.11533479895976594No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGAAAAAAAAAAAAA252410.11166726737796134No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAAAAAAAAAAAAAAAAAA252380.11165399524919727No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGGAAAAAAAAAA244610.10821651389930322No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAA239820.1060973973399734No Hit
TCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAAAAAAAAAAAAAA239430.1059248596660405No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA238290.10542051877300586No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAA234420.10370841416244085No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA231460.1023988974577193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAAAAAAAAAAAAAAAAAAA228110.10091684307906486No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAAAAAAAAAAAAAAAAAAA227920.10083278626355908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)