FastQCFastQC Report
Fri 7 Jul 2023
SRR7182549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7182549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20785991
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAA4149261.9961809855493537No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA2571681.237217893532235No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAAA1299890.6253683069525047No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAAA1254020.603300559497019No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAAA1006660.48429733275647047No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAAAAAAAAAAAAAAAAA897490.43177638246836536No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAAA866850.4170356852362728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA712590.3428222402290081No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAA699980.3367556543250692No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA605960.2915232668002214No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGATAAAAAAAAAA486370.23398932482940074No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAAA442660.21296073879758728No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA438310.21086798315269165No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA428620.2062061895437172No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAAAAAAAAA419100.20162618178753178No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAAAAAAAAAAAAAAAAAAA391590.18839130643326074No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAAAAAAAA390000.1876263681630575No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAA358120.1722891152988568No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAA349720.16824793198457558No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA344090.16553937697750373No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG337750.1624892457617248No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGATAAAAAAAAA337370.16230643032607875No Hit
TCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGAAAAAAAAAAAAAAAAA333710.1605456290248562No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAAAAAAAAAAAAGAT330970.1592274335151978No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAAAAAAAAA310020.14914852989207972No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAAA299540.14410667261426216No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGGGCGCCAAAAAAAAAAAA298130.14342833112936496No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG285020.13712119859957603No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAAAAAAAAAAAAAAAAAAAGA282740.1360243059856997No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAAAAAAAA278120.133801655162845No Hit
TCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCAAAAAAAAAAAAAAAAAA263180.12661412198244482No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTAAAAAAAAAAAAAAAAAAA262090.12608973033809165No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA256480.12339079719605384No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAA254770.12256812773564657No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGAAAAAAAAA246390.11853656628639933No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAAAAAAAAAAAAAAAAAAA245330.11802660743959717No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAAAAAAAAAAAAAAAAAA244300.11753108139034601No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA244130.117449295537557No Hit
TCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAAAAAAAAAAAAAA242540.11668435726735375No Hit
TCATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAAAAAAAA232240.11172909677484226No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAAAAAAAAAAA231180.1112191379280401No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAAAAAAA230790.11103151155987703No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCAAAAAAAAAAAAAAAAAAA230320.11080539773157796No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAAAAAAAAAAAAAAAAAAAAGAT226880.10915043694572944No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGAAAAAAAAAAAAAAAAAAA219840.10576354045376044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)