FastQCFastQC Report
Fri 7 Jul 2023
SRR7183112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7183112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40339084
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACG546003813.535354446818873No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG25083046.218048977016929No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC22417945.55737457994832No Hit
TTGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACA11819812.9301136337156293No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCAC7969861.9757166523662262No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG7256531.7988831873326623TruSeq Adapter, Index 4 (100% over 49bp)
AGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGT6085941.508695636222181No Hit
TGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACAC3398010.8423617155015221No Hit
GAGATTGAGCGATAACAGGTCCGTGATGAGATCGGAAGAGCACACGTCTG2519750.6246423443824357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTC2361140.5853231570652423No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCA2019270.5005740834372937No Hit
GACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACG1908870.4732060846993948No Hit
CGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACGT1738560.43098648447247834No Hit
GCTCAAATGCGCATTTAGTCGGGATTATGGATTAGATCGGAAGAGCACAC1610560.3992554714430303No Hit
TTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGT1586560.3933059065000088No Hit
TACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCT1557780.3861713865391688Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTC1432800.3551890270983843No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGAGATCGGAAGAGCACACGT1341640.33259059625647425No Hit
ACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCTG1286730.3189784874639196Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACA1273770.31576572239468803No Hit
CTTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACG1261180.3126446797849946No Hit
ACCGCTTGGGAACGTCGCGTGTCGTTGGCCTAGATCGGAAGAGCACACGT1183720.2934424589313927No Hit
TGGAATGAGTTGAGCATAAATCCTTCAACAAGATCGGAAGAGCACACGTC1054770.2614759422896167No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACA1024430.2539547006074804No Hit
TAGAAGATATCTGTGTGCTTAGTGGGCCAAGAGATCGGAAGAGCACACGT1023430.25370680206818774No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTC1002670.24856042839247416No Hit
GTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTC972910.24118296786312748No Hit
GAGATTGAGCGATAACAGGTCCGTGATAGATCGGAAGAGCACACGTCTGA927500.2299258951938522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG913560.22647018955611387No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAAGATCGGAAGAGCACA911170.22587771204720464No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACG871770.21611050959907765No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGT788920.1955721156186888No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAGATCGGAAGAGCACACGTCT739130.1832292473473121Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTATCTACTATCTAGCGAAACCACAGCCAGATCGGAAGAGCACACGT738280.18301853358891343No Hit
CAGTACGATGTACACATTTTTGAGTGCCTAGATCGGAAGAGCACACGTCT735150.1822426111609277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGT734680.1821260988474602No Hit
GAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTCT731000.18121383222286358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACGT720100.17851173814457463No Hit
AAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGTC712780.17669712083695305No Hit
CAGTACGATGTACACATTTTTGAGTGCCTATAGATCGGAAGAGCACACGT694840.17224982104204448No Hit
TAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGT662490.16423030329593008No Hit
ACCCTAGGCAGGGGATCACTCGGCTCGTGGATCGAGATCGGAAGAGCACA652300.16170421718053885No Hit
TTACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC651150.1614191338603524No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACG615410.1525592400660362No Hit
CGCTCAAATGCGCATTTAGTCGGGATTATGGATTAGATCGGAAGAGCACA589090.14603455051185593No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAAGATCGGAAGAGCACACGTC579560.14367207743239782No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG564240.13987427181043577No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCAGATCGGAAGAGCACAC531010.1316366033497439No Hit
TCTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACAC510610.1265794731481756No Hit
GGAATGAGTTGAGCATAAATCCTTCAACAAGATCGGAAGAGCACACGTCT480640.11914995392557749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACG468610.11616773449788795No Hit
CGCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG461440.11439030197116028No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACAC459770.1139763114105417No Hit
CCCTATCTACTATCTAGCGAAACCACAGCCAGATCGGAAGAGCACACGTC453500.11242198756917733No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGTGGAGATCGGAAGAGCACACG448430.11116514197496404No Hit
AAACTCTCAACAGCTTGTACCGAATCCGCAGAGATCGGAAGAGCACACGT417890.10359432058496916No Hit
GACCGCTTGGGAACGTCGCGTGTCGTTGGCCTAGATCGGAAGAGCACACG411810.10208709746607038No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAAAGATCGGAAGAGCACACGT411300.10196066921103117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)