FastQCFastQC Report
Fri 7 Jul 2023
SRR7183113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7183113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40081565
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACG566325014.12931356347987No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG24904676.2134974021099225No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC21063745.255218951655206No Hit
TTGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACA11860902.959190839978429No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCAC8239292.055630811820846No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG6787581.6934418603664803TruSeq Adapter, Index 2 (100% over 49bp)
AGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGT6256431.5609245796664875No Hit
TGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACAC3167190.7901862115413907No Hit
GAGATTGAGCGATAACAGGTCCGTGATGAGATCGGAAGAGCACACGTCTG2463700.6146716077578308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTC2392730.5969652133094104No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCA2118800.5286220735143451No Hit
GACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACG1883000.4697920353159863No Hit
CGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACGT1747550.43599844467150917No Hit
GCTCAAATGCGCATTTAGTCGGGATTATGGATTAGATCGGAAGAGCACAC1686920.4208717898116004No Hit
TTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGT1627650.4060844430600452No Hit
TACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCT1597240.3984974139607573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTC1443610.36016807227961284No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGAGATCGGAAGAGCACACGT1349670.3367308636776034No Hit
ACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCTG1331750.33225998036753307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACG1288530.32147696827706207No Hit
TTGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACA1264750.3155440662059977No Hit
ACCGCTTGGGAACGTCGCGTGTCGTTGGCCTAGATCGGAAGAGCACACGT1198550.2990277450493762No Hit
TGGAATGAGTTGAGCATAAATCCTTCAACAAGATCGGAAGAGCACACGTC1091990.27244195679485067No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACA1043510.2603466206970711No Hit
TAGAAGATATCTGTGTGCTTAGTGGGCCAAGAGATCGGAAGAGCACACGT1019030.2542390747467071No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTC1010320.25206600590570755No Hit
GTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTC998340.24907710065712255No Hit
GAGATTGAGCGATAACAGGTCCGTGATAGATCGGAAGAGCACACGTCTGA937640.2339329814092838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAAGATCGGAAGAGCACA927050.23129086900673662No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG899930.22452466614015695No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACG887210.22135113736202666No Hit
CAGTACGATGTACACATTTTTGAGTGCCTAGATCGGAAGAGCACACGTCT765300.19093565832571657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTATCTACTATCTAGCGAAACCACAGCCAGATCGGAAGAGCACACGT763600.19051152319027465No Hit
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGT752360.18770724147123496No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACGT740080.18464348884580728No Hit
GAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTCT730470.1822458778742796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTAGAAGATATCTGTGTGCTTAGTGGGCCAGATCGGAAGAGCACACGTCT715690.17855839710849614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTACGATGTACACATTTTTGAGTGCCTATAGATCGGAAGAGCACACGT713290.17795961809375457No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGT706540.17627555211479393No Hit
AAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGTC686670.17131816085524604No Hit
TAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGT664620.16581687865730793No Hit
ACCCTAGGCAGGGGATCACTCGGCTCGTGGATCGAGATCGGAAGAGCACA644840.16088194161081285No Hit
CGCTCAAATGCGCATTTAGTCGGGATTATGGATTAGATCGGAAGAGCACA627460.15654578357905935No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACG624480.15580229963575526No Hit
TTACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC610400.15228946274927138No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAAGATCGGAAGAGCACACGTC568190.1417584368275041No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG561370.1400569064606135No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCAGATCGGAAGAGCACAC547710.13664885590170942No Hit
TCTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACAC507110.1265195109023313No Hit
GGAATGAGTTGAGCATAAATCCTTCAACAAGATCGGAAGAGCACACGTCT498430.12435392679901594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACG480120.1197857418990501No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACAC467650.11667458593495539No Hit
CCCTATCTACTATCTAGCGAAACCACAGCCAGATCGGAAGAGCACACGTC460740.11495060135501196No Hit
CGCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG458230.11432437830209476No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGTGGAGATCGGAAGAGCACACG451990.11276755286376668No Hit
TGCAGGAACCTCGCGGAACCTGTGCAATTATTAGTAGATCGGAAGAGCAC415760.10372848465373045No Hit
GACCGCTTGGGAACGTCGCGTGTCGTTGGCCTAGATCGGAAGAGCACACG415550.10367609148994056No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACAGATCGGAAGAGCACACGT409140.10207685253806832No Hit
AAACTCTCAACAGCTTGTACCGAATCCGCAGAGATCGGAAGAGCACACGT407450.1016552123151878No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAAAGATCGGAAGAGCACACGT404960.10103397908739341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)