FastQCFastQC Report
Fri 7 Jul 2023
SRR7183114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7183114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43887839
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACG35454888.078520339085276No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG31305717.133117217277434No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC22471155.1201313420786105No Hit
TTGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACA15197473.4627975189209024No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCAC11901102.7117079061468488No Hit
TGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACAC4515671.028911448567791No Hit
AGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGT3903930.8895243167475163No Hit
GAGATTGAGCGATAACAGGTCCGTGATGAGATCGGAAGAGCACACGTCTG3660160.8339804564084371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACG3567140.8127855190135927No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTC2504930.570757197682939No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCA2402190.5473475237639293No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGAGATCGGAAGAGCACACGT2326480.5300967313519356No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2182040.49718556431999306TruSeq Adapter, Index 6 (100% over 49bp)
TTGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACA2103750.47934690974417765No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACA1814700.413485840576475No Hit
TGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTC1652460.37651888032126624No Hit
TACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCT1611710.36723384808260895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACGT1547550.35261476419470095No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGT1527550.34805769315732316No Hit
ACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCTG1497460.3412015797815882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTC1489460.33937875136663714No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACG1422500.32412167753349624No Hit
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGT1355790.3089215670883226No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACGT1283480.2924454767526831No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAAGATCGGAAGAGCACA1245470.2837847632461466No Hit
GACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACACG968150.22059641624186602No Hit
GAGATTGAGCGATAACAGGTCCGTGATAGATCGGAAGAGCACACGTCTGA967640.22048021093041287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACG966420.22020222959713281No Hit
TTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGT910290.20741280973073203No Hit
GTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTC850150.19370969712133695No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACAC814460.18557760385513627No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCAGATCGGAAGAGCACAC776190.17685764842511387No Hit
AAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGTC731680.16671588683142954No Hit
CTTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACG707470.16119955234068373No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGTGGAGATCGGAAGAGCACACG670480.15277124945705348No Hit
TAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGT663230.15111931120600403No Hit
TTACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC646610.14733238517394306No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGTC643720.14667388840904197No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACAC642690.14643919925061702No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTCT632090.14402395160080678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACG576770.13141909311141978No Hit
GAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTCT563450.12838408380052616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAACCGCATAAGGCTCAGTATAACAGCAGATCGGAAGAGCACACGTC539200.12285863516770558No Hit
CAGTACGATGTACACATTTTTGAGTGCCTAGATCGGAAGAGCACACGTCT539200.12285863516770558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACAC534450.12177633079632832No Hit
TCGATCCTCTCAGACTACCCTTGCTTGGAGATCGGAAGAGCACACGTCTG533710.12160771916794537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTACGATGTACACATTTTTGAGTGCCTATAGATCGGAAGAGCACACGT511850.11662684052409142No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG511070.11644911475363368No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACG507950.11573821167180276No Hit
AAACTCTCAACAGCTTGTACCGAATCCGCAGAGATCGGAAGAGCACACGT501720.11431868404365955No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG501150.11418880751909431No Hit
AGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTCTG470240.1071458542308269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCAC450950.10275055921527601No Hit
AGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTCTGAA440440.10035581838513398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)