FastQCFastQC Report
Fri 7 Jul 2023
SRR7183115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7183115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38735336
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACG531088113.710687832938895No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG24360446.288945060396534No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC23027005.944701241264565No Hit
TTGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACA11752613.0340797869934577No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCAC7697431.9871855506816825No Hit
AGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGT5923781.5292961444816173No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG5863061.5136205350071057TruSeq Adapter, Index 5 (100% over 49bp)
TGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACAC3518650.9083824650443202No Hit
GAGATTGAGCGATAACAGGTCCGTGATGAGATCGGAAGAGCACACGTCTG2371450.612218776158286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTC2315380.5977436209666542No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCA1945140.5021616438282606No Hit
GACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACG1897210.4897879290371975No Hit
CGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACGT1625480.41963751134106597No Hit
TTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGT1552040.40067807853790144No Hit
GCTCAAATGCGCATTTAGTCGGGATTATGGATTAGATCGGAAGAGCACAC1536860.3967591761692734No Hit
TACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCT1515870.3913403513525738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTC1364960.3523810920344153No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGAGATCGGAAGAGCACACGT1283050.33123502530092935No Hit
TTGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACA1270510.3279976711703237No Hit
ACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCTG1244810.32136290233806153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACG1230850.3177589578673075No Hit
ACCGCTTGGGAACGTCGCGTGTCGTTGGCCTAGATCGGAAGAGCACACGT1155240.2982393130654656No Hit
TGGAATGAGTTGAGCATAAATCCTTCAACAAGATCGGAAGAGCACACGTC1036030.2674637958478016No Hit
TAGAAGATATCTGTGTGCTTAGTGGGCCAAGAGATCGGAAGAGCACACGT1009000.2604856712744147No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACA990460.2556993438755766No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTC973010.251194413287134No Hit
GTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTC949980.24524893755923532No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAAGATCGGAAGAGCACA911410.23529162106661472No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG901810.23281326383744289No Hit
GAGATTGAGCGATAACAGGTCCGTGATAGATCGGAAGAGCACACGTCTGA896460.23143209600660236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACG850400.21954114455080498No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGT831410.21463864415684947No Hit
AAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGTC754700.19483502092249827No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAGATCGGAAGAGCACACGTCT722420.18650154473940797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTACGATGTACACATTTTTGAGTGCCTAGATCGGAAGAGCACACGTCT720670.18604976086950686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGT719070.18563670133131155No Hit
TAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGT714720.18451369571184306No Hit
TCCCTATCTACTATCTAGCGAAACCACAGCCAGATCGGAAGAGCACACGT712030.1838192393632522No Hit
GAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTCT708860.18300086515320274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACGT702410.18133571888985292No Hit
CAGTACGATGTACACATTTTTGAGTGCCTATAGATCGGAAGAGCACACGT680330.1756354972627577No Hit
TTACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC662430.1710143936791977No Hit
ACCCTAGGCAGGGGATCACTCGGCTCGTGGATCGAGATCGGAAGAGCACA637530.1645861546160333No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACG586150.15132178019573653No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAAGATCGGAAGAGCACACGTC562450.14520333578621855No Hit
CGCTCAAATGCGCATTTAGTCGGGATTATGGATTAGATCGGAAGAGCACA557100.143822167955378No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG546780.14115793393401826No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCAGATCGGAAGAGCACAC525230.13559453827895027No Hit
TCTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACAC503430.12996660207103922No Hit
GGAATGAGTTGAGCATAAATCCTTCAACAAGATCGGAAGAGCACACGTCT469900.12131042312373386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACG454510.11733730669071774No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACAC447820.11561020149663861No Hit
CCCTATCTACTATCTAGCGAAACCACAGCCAGATCGGAAGAGCACACGTC439000.113333210792337No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGTGGAGATCGGAAGAGCACACG438090.11309828317998842No Hit
AAACTCTCAACAGCTTGTACCGAATCCGCAGAGATCGGAAGAGCACACGT424630.10962341981492041No Hit
GACCGCTTGGGAACGTCGCGTGTCGTTGGCCTAGATCGGAAGAGCACACG399180.10305319153550133No Hit
GTAGAAGATATCTGTGTGCTTAGTGGGCCAAAGATCGGAAGAGCACACGT397370.1025859179329179No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)