FastQCFastQC Report
Thu 6 Jul 2023
SRR7183116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7183116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34043447
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACG28112028.257689064212563No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG24402567.168063798004944No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC17807815.230906846771422No Hit
TTGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACA11992793.5227895694581104No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCAC9528242.7988470145223543No Hit
TGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACAC3592711.055330854128843No Hit
AGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGT3066760.9008370979589699No Hit
GACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACG2821460.8287821148075868No Hit
GAGATTGAGCGATAACAGGTCCGTGATGAGATCGGAAGAGCACACGTCTG2745030.8063313917653522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2146610.6305501320121902TruSeq Adapter, Index 12 (100% over 49bp)
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTC1972160.5793067899381634No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCA1947080.5719397333648382No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGAGATCGGAAGAGCACACGT1832590.5383091788560659No Hit
TTGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACA1668600.4901383811104674No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACA1433500.4210795692927335No Hit
TGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTC1291800.37945628713802104No Hit
TACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCT1257510.3693838640957832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGT1202260.35315460270518434No Hit
CGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACGT1188750.34918614439953743No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTC1163760.34184552463209733No Hit
ACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCTG1153090.33871129442327036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACG1116570.32798382607965637No Hit
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGT1063380.31235967380153956No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACGT996720.2927788129092803No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAAGATCGGAAGAGCACA975260.28647510341711285No Hit
GACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACACG777760.22846100161361452No Hit
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACG757640.222550906786848No Hit
GAGATTGAGCGATAACAGGTCCGTGATAGATCGGAAGAGCACACGTCTGA733810.21555102807303853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGT719950.21147975996672724No Hit
GTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTC667800.19616109966772755No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACAC646340.18985739017556008No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCAGATCGGAAGAGCACAC610530.1793384788561511No Hit
AAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGTC577780.1697184189368368No Hit
CTTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACG560510.16464548962976633No Hit
TAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGT522270.1534127845514586No Hit
TTACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC520710.15295454658278287No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGTGGAGATCGGAAGAGCACACG520470.15288404843375583No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACAC504490.14819004667770572No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGTC497990.1462807218082235No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTCT492590.14469451345511516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACG456250.13401991872327146No Hit
GAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTCT434480.1276251491219441Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAACCGCATAAGGCTCAGTATAACAGCAGATCGGAAGAGCACACGTC426180.1251870881347591No Hit
CAGTACGATGTACACATTTTTGAGTGCCTAGATCGGAAGAGCACACGTCT420580.12354213132412825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACAC414350.12171211687230146No Hit
TCGATCCTCTCAGACTACCCTTGCTTGGAGATCGGAAGAGCACACGTCTG410020.12044021276693868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG405140.11900675040338894No Hit
CAGTACGATGTACACATTTTTGAGTGCCTATAGATCGGAAGAGCACACGT404300.1187600068817943No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACG394600.11591070669195161No Hit
AAACTCTCAACAGCTTGTACCGAATCCGCAGAGATCGGAAGAGCACACGT393210.11550240491217004No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG386780.11361364200282069No Hit
AGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTCTG368890.10835859247743038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCAC357490.10500993039864617No Hit
AGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTCTGAA345120.10137633830087771Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)