FastQCFastQC Report
Fri 7 Jul 2023
SRR7183117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7183117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40960959
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACG33354908.143095477818282No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG28666656.9985299904721465No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC21090205.148854058812442No Hit
TTGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACA14659203.5788224587222186No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCAC11189922.7318501014588064No Hit
TGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACAC4259071.039787667080744No Hit
AGTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGT3657030.8928086864372486No Hit
GACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACG3404120.8310645265898194No Hit
GAGATTGAGCGATAACAGGTCCGTGATGAGATCGGAAGAGCACACGTCTG3390050.8276295484195084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2353500.5745715084453955TruSeq Adapter, Index 7 (100% over 49bp)
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTC2328430.5684510462755522No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCA2239820.5468182519847741No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGAGATCGGAAGAGCACACGT2162000.5278196733626281No Hit
TTGACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACA2045300.4993291294766804No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACA1691900.413051852618978No Hit
CGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACGT1548210.37797210753781424No Hit
TGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTC1532750.3741977818439261No Hit
TACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCT1512750.3693150836629582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTCTG1410570.3443693786563933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTC1407320.343575940201986No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGT1386110.33839783878106955No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACG1333120.3254611299505951No Hit
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGT1274760.31121341665853086No Hit
TGACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAAGATCGGAAGAGCACA1222350.29841830607530456No Hit
AGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACGT1185960.2895342367350335No Hit
GACGCCGGGTCGCCCCCCTGCCAGGGTGTGCCAGATCGGAAGAGCACACG935780.2284565651893062No Hit
GAGATTGAGCGATAACAGGTCCGTGATAGATCGGAAGAGCACACGTCTGA894660.21841773772923626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACACG888450.21690165994404575No Hit
TTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGT847430.20688724597488062No Hit
GTACGATGTACACATTTTTGAGTGCCTATAAGATCGGAAGAGCACACGTC793960.19383335238806298No Hit
AAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGTC772330.18855271430534623No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACAC767300.1873247157128328No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCAGATCGGAAGAGCACAC721830.17622390139840233No Hit
TAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACGT693580.1693270902177852No Hit
CTTGAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACG652850.1593834753722441No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGTGGAGATCGGAAGAGCACACG618780.1510657990209653No Hit
TTACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACAC610690.14909074760676377No Hit
CTAAGGTCGTAACCAAACCGAGCTGATAGTGTAGATCGGAAGAGCACACG607590.14833392938871376No Hit
CCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACGTC593280.14484035884023125No Hit
CCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCACAC587860.14351714763318896No Hit
TCCCAGAGAGGGTGATAGGCCCGTAGAGCAGATCGGAAGAGCACACGTCT587600.1434536725568364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACACG528820.12910342260297178No Hit
GAACAAATTAGAGTGCTCTAAGCAGGCTAAGATCGGAAGAGCACACGTCT518070.12647897233070152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAACCGCATAAGGCTCAGTATAACAGCAGATCGGAAGAGCACACGTC503580.12294145749859031No Hit
CAGTACGATGTACACATTTTTGAGTGCCTAGATCGGAAGAGCACACGTCT500890.12228473459325012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATCCTCTCAGACTACCCTTGCTTGGAGATCGGAAGAGCACACGTCTG500570.12220661142235464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGAGATCGGAAGAGCACAC492730.12029259373541523No Hit
ACGAACGTGACTCAATCAAATTAAATAGAACGAGATCGGAAGAGCACACG483030.11792448511764579No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG482930.11790007162674097No Hit
CAGTACGATGTACACATTTTTGAGTGCCTATAGATCGGAAGAGCACACGT478820.11689667715055208No Hit
AAACTCTCAACAGCTTGTACCGAATCCGCAGAGATCGGAAGAGCACACGT471500.11510960961631782No Hit
CCAGAAGGCCCGGGTTCGATTCCCGGTATGGGAGATCGGAAGAGCACACG451810.11030259325715494No Hit
AGAGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTCTG446000.10888416943558377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTGATAGGCCCGTAGAGCGGCGCAGATCGGAAGAGCACACGTCTGAA421920.10300540082569844Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCAAGATCGGAAGAGCAC419750.10247562807306342No Hit
ACGCCGGGTCGCCCCCCTGTCAGGGTGTGCCAGATCGGAAGAGCACACGT411260.10040292269524256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)