FastQCFastQC Report
Fri 14 Jul 2023
SRR7215496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7215496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81029804
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length81
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC24112102.9757075557038246No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA18704692.308371620891493No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT18305412.2590959247538103No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT16015391.9764813944261792No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA12832071.5836234776033766No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG11182251.38001691328292No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG10557811.3029539106376216No Hit
TCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCG9554901.1791834026897066No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT9145331.128637803443286No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA8144651.0051425028746213No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGG7689040.9489150436548013No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC7510730.9269095603390575No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCACCATCAA6926690.8548323774792791No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA6736760.8313928539182941No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC6197250.7648111798468623No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATCG6129100.7564006942433181No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT5909510.7293007891269242No Hit
CGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGGA5667750.6994648536975359No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA5519030.6811111131405427No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA5197130.6413849896514621No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA5038340.621788496489514No Hit
TCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGG4812060.5938629692353693No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG4777850.5896410658971852No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCACCATCAAT4561050.5628854785333061No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4281530.5283895293637881No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA4146360.5117080130170376No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT3606350.44506463325519086No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCTGTAGGCACCATCAATAGATCG3366240.41543232660417145No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA3229320.3985348403409689No Hit
CCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC3202960.3952817163422979No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA3158320.3897726323020601No Hit
TTGGCGCGCGGGCGGGGCCGGGGGTGGGGCTGTAGGCACCATCAATAGAT3064340.3781744307316849No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2872520.35450165966093167No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG2753440.33980583243173096No Hit
TTGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAG2706280.3339857517118022No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG2695160.332613417156976No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA2520070.3110053184875037No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2513770.3102278267882766No Hit
TCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA2448780.30220732114815435No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA2444400.3016667793001202No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCTGTAGGCACCATCAATAGAT2397780.2959133407258396No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA2387930.294697738624667No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA2263410.27933055348375274No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC2189020.2701499808638313No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG2138260.2638856191729157No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC2092320.25821610033759923No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGAT2049950.2529871601318448No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA2033260.2509274241857971No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA2006940.24767923664235938No Hit
TCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCGG1947030.24028566081685204No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG1872430.2310791718069564No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAA1865080.23017209815785808No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1757060.2168412008006338No Hit
GCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1748230.21575147830790753No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA1726920.21312158178242663No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1707120.21067803644199853Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG1694110.20907245437740413No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGCTGTAGGCACCATCAATA1689940.2085578289193443No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGAT1549480.19122346636800455No Hit
GTCCCGCGCGTGTGGGGGGACTCCGCGTCGGTCTGTAGGCACCATCAATA1523100.18796787414171703No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCGGA1509800.18632650277668203No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAG1475620.18210830177992285No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1463270.18058417122667605No Hit
TTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATAGAT1420620.1753206758342893No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCAATAGAT1399070.17266116057740927No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCTGTAGGCACCATCAATAGATC1395360.17220330435453107No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG1371220.1692241536213021No Hit
TCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAA1370680.16915751147565408No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1364280.16836767863834398No Hit
GTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGATCGGAAGAGC1352500.1669138925721701No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1338890.1652342636790779No Hit
CCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCG1280500.15802827315243168No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1258030.15525521942518827No Hit
TTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCTGTAGGCACCATCAATAGA1233130.15218227604252876No Hit
TGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAGA1197660.14780487436449927No Hit
ACCCACGGCTGTCTCTCCAGCCACCTTTTTCTGTAGGCACCATCAATAGA1178740.14546993103920133No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA1177910.14536749959311268No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1154460.14247350271265619No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG1153370.14233898430755182No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1138770.1405371781474382No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1129420.1393832817366805No Hit
ACGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1118570.13804426825467825No Hit
TCGGCGCCCGGCCCCGTCCTCGCGTCGGGGTCGGGGCTGTAGGCACCATC1116650.1378073184034852No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1092920.13487876633639642No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGA1088090.13428268936698898No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGCTGTAGGCACCATCAATAGATCGG1086540.13409140172670292No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1063450.13124183294334515No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1058200.13059392319398921No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1046410.12913890301400704No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGA1017090.12552048132808022No Hit
TCACCCACGGCTGTCTCTCCAGCCACCTTTTTCTGTAGGCACCATCAATA1011800.1248676351234911No Hit
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCACCATCAAT967900.11944987550506724No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA961360.118642765074441No Hit
GTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA954740.11782578173334839No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGCTGTAGGCACCATCAATAGAT953660.11769249744205232No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG944750.11659290203885969No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC929760.11474296544022246No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCACCATCAATAGA906440.11186501203927385No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT902140.11133434310170613No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATC901470.11125165747655023No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCTGTAGGCACCATCAATAGATCGG890360.10988055703553226No Hit
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAA877110.10824535623953874No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA867720.10708652337354783No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG857470.10582155672004341No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG845150.1043011285082215No Hit
GCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG839790.1036396435069743No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG816980.10082462991024882No Hit
GTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATCGGAAGAGCA814350.10050005797866671No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT812400.1002594057860488No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAG811800.10018535895755097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)