FastQCFastQC Report
Fri 7 Jul 2023
SRR7215497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7215497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59590155
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length81
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA40799626.846704795448175No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT24670824.140083206697482No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC23706153.9781990833888585No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA21726013.6459059386571493No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA20584843.4544028287894197No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA20108893.374532252852841No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA9945141.6689233313791514No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC9539491.6008500061797122No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG7232891.2137726441557333No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG5133150.8614090700049362No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG4007950.6725859330287025No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA3523380.5912688094199453No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3266470.5481559831485586No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3073290.5157378764999017No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG2981360.5003108315459156No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA2969690.49835245436095943No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAG2797170.46940136336279037No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2610370.43805390336709144Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG2479850.4161509564793044No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA2478810.4159764310060949No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG2425810.40708234439061286No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC2405790.4037227290313307No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA2273590.3815378563791284No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGCTGTAGGCACCATCAATA2206510.3702809633571183No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCTGTAGGCACCATCAATAGAT2030890.340809652198421No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG1796090.3014071703622855No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1770580.2971262618800035No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA1692220.28397643872549083No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG1690980.28376835066127953No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCCTGTAGGCACCATCAATAGA1656670.27801068817491753No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1624880.27267591433517835No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1459990.24500523618372866No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1420600.2383950838859204No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA1396000.2342668851926967No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGAT1384050.232261520380338No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATAGA1331540.22344966211281042No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC1325990.22251830021250996No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1312810.22030652546549007No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT1306220.21920063809198012No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG1286550.21589975726695124No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1278570.21456060988597866No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1237410.2076534286578043No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC1212920.20354368938963158No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGAT1208460.20279524361029097No Hit
TTGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAG1203990.20204511970140035No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1199290.20125639881285762No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1197260.20091573851418912No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA1153220.1935252559755886No Hit
GTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA1145590.1922448431288692No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA1110510.18635796466715013No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGCTGTAGGCACCATCAATAG1098810.18439455309354372No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGCTGTAGGCACCATCAATA1096130.18394481437411936No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG1093810.18355548831849824No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1065530.17880973795084104No Hit
ATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGCTGTAGGCACCATCAA1049750.17616164952079752No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1049530.17612473067069553No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT1049450.1761113056342948No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1034570.17361424886375945No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA1015020.17033350559333163No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATC981430.16469666843457614No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT962210.16147130343930136No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGA940840.15788514059075698No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT931740.1563580427001742No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA928180.15576062858034181No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT925890.155376336913371No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATA893940.15001471300083044No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAGA887100.14886687238856822No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA816280.1369823589148241No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG761540.12779627775762623No Hit
TCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGATCGGAAG758350.12726095443114724No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA741090.12436450282769025No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCCTGTAGGCACCATCAATAGATC735650.12345160035244077No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT722830.12130023826922418No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT717850.12046452975327888No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAG715240.12002653794070514No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAG695650.11673908215207697No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGCTGTAGGCACCATCAATAG695050.1166383943790715No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAGA693380.11635814674420633No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG678060.11378725227346699No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGA670820.11257228647920114No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA666930.11191949408421577No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT662410.11116097952757464TruSeq Adapter, Index 5 (100% over 43bp)
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCTGTAGGCACCATCAAT635270.10660653592862782No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAATA633550.10631789764601217No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT630780.10585305576063697No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAG619410.10394502246218355No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC612810.10283745695912354No Hit
GTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC600550.10078007013071204No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG598770.10048136307079583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)