FastQCFastQC Report
Fri 7 Jul 2023
SRR7215498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7215498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79426541
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length81
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA44933995.6573016317052005No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC31508423.9669888180073207No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA30016813.7791913914518824No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT25862293.2561269412449927No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA20998702.643788806061692No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA16655012.0969073801161757No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG11185891.408331504704454No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA10600841.334672247655856No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC7485620.9424582646750285No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG5092160.6411156693831096No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA4592820.5782475155250686No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG4568950.5752422228735857No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG4400610.5540477961894376No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA4216450.5308615919708753No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT3908860.49213524229891864No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA3695730.4653016426838983No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA3438580.43292581506224725No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG3407230.4289787717181338No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG3245000.40855360930296586No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAG3203050.4032719994692958No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG3044060.3832547611509357No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2972350.37422629294658566No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG2921050.36776749474712744No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC2825330.35571610754143257No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG2734890.34432948553053566No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGCTGTAGGCACCATCAATA2715000.3418252848251317No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG2631150.33126836028274226No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCTGTAGGCACCATCAATAGAT2325730.2928152190336477No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA2233600.2812158217994159No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATC2163460.2723850205185191No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA2134660.26875902854689343No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCCTGTAGGCACCATCAATAGA2130670.26825667757582444No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1975830.24876193462837567No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG1969750.2479964474343658No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGAT1953690.2459744533001884No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT1939780.2442231495388928No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1929190.2428898420743263No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATA1923180.24213316805524743No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1913350.2408955464899321No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1899070.23909765880400102No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGAT1893130.23834979795985325No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1773950.2233447381272716No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGCTGTAGGCACCATCAATA1740630.21914966686009907No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1720740.2166454661546951No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC1712770.21564202323754728No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAG1701890.2142722040482664No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT1694420.213331712380626No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1688360.21256874323659644No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGA1646030.20723929045330072No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1586050.19968765856239415No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1541240.1940459675815418No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG1416920.17839376890402414No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT1393380.1754300240772162No Hit
TTGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAG1359020.1711040142110683No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1342940.16907950202691063No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA1289870.16239785640419616No Hit
TCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAA1273940.16039222959489072No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAA1209260.15224885595861462No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA1194290.15036409554836339No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1173800.1477843533435505No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1163900.14653791860330415No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATAGAT1144270.144066452547644No Hit
TCGCGCCGAACCACCTCACCCCGAGTGTTACCTGTAGGCACCATCAATAG1124780.1416126128418459No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1110920.13986760420550104No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1104970.1391184843363631No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATAGA1075620.13542324599027925No Hit
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTCTGTAGGCACCATCAATAG1056610.13302983948400823No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1051590.13239780893895403Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGA1003980.1264035909608603No Hit
GTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA998540.1257186813662199No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAGA990300.12468124477433809No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT984130.12390442635541689No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG978120.12314775233633805No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCCTGTAGGCACCATCAATAGATC974910.12274360531450061No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA963740.1213372744005055No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA960500.1209293503036976No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG932030.11734490615674677No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA928240.11686773568548076No Hit
TCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGG890080.1120632963230767No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCCTGTAGGCACCATCAATAGA865450.10896231777234264No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCTGTAGGCACCATCAATAGATCG845140.10640523801735242No Hit
TTCCCGCGCGTGTGGGGGGACTCCGCGTCGGTCTGTAGGCACCATCAATA839740.1057253645226726No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT820940.1033583975411947No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA809120.10187023000284No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAG808690.10181609192826363No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC797970.10046641713882516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)