FastQCFastQC Report
Fri 14 Jul 2023
SRR7215499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7215499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61574286
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length81
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA30767794.996856967208682No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA25728924.178516986782437No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC19416803.1533942594153674No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA18616533.0234260450864183No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT18600383.0208031969708915No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA13022282.1148893224681484No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG8885831.4431072737083788No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA7298311.1852853640885093No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC6236481.0128383786699533No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG4929180.8005257259499526No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4567350.7417625597802303No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA4558890.7403886096218801No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG4075820.66193540595826No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG4011910.6515560732608414No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT3857310.6264481897524561No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA3718650.6039290492138227No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG3162410.513592638329578No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT2726310.44276761893755456No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC2272540.3690728951367783No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG2104260.34174330498935873No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG2097490.3406438200517664No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA1841720.2991053765528032No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1832760.29765022366641813No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC1810580.2940480706507908No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1805700.2932555320251704No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1778230.288794254146934No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1762660.2862656011959278No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA1740460.28266020007117904No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAG1700270.2761331247917353No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG1682780.2732926533650752No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1633100.26522435030752933No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCCTGTAGGCACCATCAATAGA1625310.2639592118047459No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1597060.2593712576707751No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCTGTAGGCACCATCAATAGAT1590710.2583399830247321No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGAT1583070.2570992053403591No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATC1542690.2505412730242621No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA1514410.24594844672660923No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGCTGTAGGCACCATCAATA1512830.245691846106019No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG1491310.24219688069139772No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1439080.233714443720874No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA1301230.2113268516016572No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGA1293240.21002923200765983No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1264600.20537793974582183No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT1261170.20482088903150253No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1214200.1971927047599058No Hit
TTGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAG1192480.19366525825407055No Hit
TCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAA1189880.19324300406828915No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG1189010.19310171132150847No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1062090.1724892108371342No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1041400.1691290419510508No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1033910.16791262508508828No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1030520.16736207058901179No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1026580.16672219309209693No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA1022320.16603034584923973No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1018120.16534824293374673No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA990360.16083986747325013No Hit
TCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGG972540.15794580224608695No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA971510.15777852462633507No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT961030.15607651544672396No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT937350.152230754246992No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGAT926570.15048002343055997No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA875860.14224444275326228Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGA874410.14200895484196113No Hit
CGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGGA866270.14068697442955327No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT857120.13920096450651492No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG855650.1389622284860924No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATA803580.13050577638853986No Hit
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCAT793430.12885736100943176No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA785720.1276052149431339No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT780160.12670224060738602No Hit
TTCCCGCGCGTGTGGGGGGACTCCGCGTCGGTCTGTAGGCACCATCAATA780070.12668762411633974No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAG777080.12620203180269116No Hit
TCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA771050.12522272690259048No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGCTGTAGGCACCATCAATA761060.12360029639645355No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA721420.11716254411784814No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA715920.11626931410946446No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG689370.11195744925081225No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCCTGTAGGCACCATCAATAGATC680050.11044383040024208No Hit
GTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA674440.10953273579169072No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA667240.10836341650798842No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG652500.10596956008552011No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA650040.10557004266358849No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCTGTAGGCACCATCAATAGATCG638340.10366989882757227No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG625250.10154401140761908No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG623970.10133613242384977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)