FastQCFastQC Report
Fri 14 Jul 2023
SRR7215502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7215502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63189766
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length81
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA26585814.207296795496916No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA13727732.1724609646441797No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT13300062.104780701355976No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA12373451.9581414496771519No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA11261081.7821050326408867No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT11173941.7683148249037668No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC11126801.760854756132504No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT9758741.5443545082917383No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC9268101.4667090237365334No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG9178341.45250419189715No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA6756561.0692490932788072No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG5711360.903842562100958No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG5000060.7912768659406019No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4983160.7886023822275271No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG4955700.7842567418274662No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA4913040.7775056486203794No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA4912860.7774771629950331No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC4199530.6645902122821598No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA3222570.5099828981800629No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA3212780.5084335966681693No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATCG3090920.4891488283086853No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT3083490.4879730049957773No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG2960780.4685537211832689No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG2672230.42288968121831627No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC2624500.41533624289730714No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA2586570.409333688622933No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGG2574420.40741090891205395No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA2478410.39221699285925504No Hit
TCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCG2468970.3907230800633128No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGAT2403040.3802894285128386No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGAT2382890.377100621008788No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA2296330.363402200286673No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2268140.3589410348504851No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2170710.3435223988643984No Hit
TTGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAG2170480.3434860005653447No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCTGTAGGCACCATCAATAGAT1967380.31134471996620466No Hit
CGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGGA1892060.2994250682934955No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1881900.29781721299616776No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGA1806330.285857997954922No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1794200.2839383833135258No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1708270.270339662280123No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1679240.26574556392565213No Hit
GTCCCGCGCGTGTGGGGGGACTCCGCGTCGGTCTGTAGGCACCATCAATA1551820.24558090624991394No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1548330.2450286016251429No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1506510.23841044133633918No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT1505320.2382221197021049No Hit
TCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATAGATCGG1467110.2321752544549698No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGCTGTAGGCACCATCAATA1445040.2286826002805581No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1413020.22361532403838935No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC1411720.22340959452199902No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGA1399990.22155328127026142No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1390550.2200593684743191No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCACCATCAAT1389680.21992168795181172No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAG1385950.2193314024932455No Hit
CCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1371960.21711743638993694No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAG1368810.21661893794637566No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1348680.213433295511808No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1345530.21293479706824678No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCACCATCAA1334880.2112493975685873No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1318730.2086936039611224Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATC1293950.20477208287177387No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1231430.19487807566813906No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1213650.1920643288978155No Hit
ACCCACGGCTGTCTCTCCAGCCACCTTTTTCTGTAGGCACCATCAATAGA1190970.18847514010417446No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA1098300.17380979065502475No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG1084060.17155626118317957No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAG1077200.17047064235053505No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1056360.16717263994932344No Hit
CCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1014840.16060195570276364No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCTGTAGGCACCATCAATAGATCG992340.15704125253446896No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG990700.1567817168368688No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA969970.1535011223178133No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC948040.1500306236297821No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG922260.1459508490662871No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC893910.14146436307423577No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT876050.13863795602598053No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCAATAGAT868310.13741307413608717No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA857870.13576090786599843No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA845770.13384604082882662No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG840600.1330278703674896No Hit
TGGGCGAGTGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAGA815890.12911742702133128No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA800150.12662651733826644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC791830.12530984843336815No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA780840.12357064275249888No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA775210.12267967569305448No Hit
CTGTTCAGAGTTCTACAGTCCGACGATCAGCTGTAGGCACCATCAATAGA745420.11796530469823231Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCCTGTAGGCACCATCAATAGATC741810.11739400965656369No Hit
TCACCCACGGCTGTCTCTCCAGCCACCTTTTCTGTAGGCACCATCAATAG739710.1170616773608562No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATC739260.1169904632974903No Hit
TCACCCACGGCTGTCTCTCCAGCCACCTTTTTCTGTAGGCACCATCAATA733460.116072593147441No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATAGAT715750.1132699241203077No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA706700.11183773017928252No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCCTGTAGGCACCATCAATAGATC678940.10744461373697758No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCCTGTAGGCACCATCAATAGA675490.10689863925117242No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGA671730.10630360618838185No Hit
TCTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGCTGTAGGCACCA670030.1060345752823329No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA664430.10514835582711289No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG662440.10483343141356151No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT659070.10430011720568802No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGACTGTAGGCACCATCAATAGAT655040.1036623557048779No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG653570.10342972309788266No Hit
TCGCCGCGCTCTCTCTCTCCCCCCGTCCGCCCTGTAGGCACCATCAATAG645490.1021510350267795No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCTGTAGGCACCATCAATAGAT634540.10041815948487608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)