FastQCFastQC Report
Fri 7 Jul 2023
SRR7235648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39980554
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4335671.0844447027922623No Hit
AAACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1176130.294175513425852No Hit
GGGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG1073820.2685855728762538No Hit
CCGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC1022890.2558468799606929No Hit
GCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG828430.20720823428309673No Hit
CCGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC810190.20264601636085386No Hit
GGGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC731690.1830114710266396No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG694650.17374696708804985No Hit
GGGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC690030.1725914053116923No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA679490.16995512368337867Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA676620.16923727470109592Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC667230.16688863290888867No Hit
CCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG662860.16579560153168463No Hit
CGGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC625680.1564960805695689No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC620800.15527548717809161No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT609130.15235656814560397No Hit
GCGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC607990.15207142952546382No Hit
GCCGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAA584150.14610853066218144No Hit
CCGCGCCGCGACCGGCTCCGGGACGGCCGGAAAAAAAAAAAAAAAGATCG581450.1454332023513231No Hit
CCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG572120.14309956785491265No Hit
CGGCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC562650.14073091633497625No Hit
CCCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC562110.14059585067280458No Hit
GGGAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAA547470.13693407049837278No Hit
CGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG545150.13635378839422785No Hit
AAATCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA525200.13136386254177468No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA523290.13088613029224158Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG514710.12874008699329179No Hit
CGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC506180.1266065497741727No Hit
GGGCGCCGCGACCGGCTCCGGGACGGCCGGAAAAAAAAAAAAAAAGATCG505770.1265039999195609No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG505410.1264139561447798No Hit
CGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG492600.12320989849215196No Hit
CCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG489980.12255457990902277No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG476210.11911040552364531No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG474020.11856263922706024No Hit
GCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG472630.1182149702077665No Hit
CCGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT468970.11729952516415856No Hit
GCGCGCCGCGACCGGCTCCGGGACGGCCGGAAAAAAAAAAAAAAAGATCG468230.11711443518266405No Hit
ATCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA463770.11599889286176475No Hit
GGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG460380.11515098064924262No Hit
CCCGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAA453600.1134551562241984No Hit
AATCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA447020.111809356118477No Hit
CGCCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC445980.11154922965799824No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG445580.11144918101935256No Hit
GGCCGCCGCGACCGGCTCCGGGACGGCCGGGAAAAAAAAAAAAAAAGATC441760.11049371652028633No Hit
CCCCGCCGCGACCGGCTCCGGGACGGCCGGAAAAAAAAAAAAAAAGATCG433390.10840019875662553No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAGAT430780.1077473813894625No Hit
TGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA411910.10302758686135263No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA411560.10294004430253768No Hit
GCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG408810.10225220991184865No Hit
CGCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG401840.10050886238344771No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)