FastQCFastQC Report
Fri 7 Jul 2023
SRR7235649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22679423
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6529622.8790944108234147No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2255570.9945447024820694Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2185170.9635033483876552Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1765950.7786573758953215Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1062820.4686274425941083Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA928880.40956950271618464Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC865780.38174692539576516Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC783800.34559962129548005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC780400.3441004649897839Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG758280.3343471304362549No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA726350.3202682890124674Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA606610.2674715313524511Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA571730.25209195136930956Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG569840.25125859683467255No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG536730.23665945998714344No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA518270.22851992310386376Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA481240.21219234722153207No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA472440.20831217795973028No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA467030.20592675572037258Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG449010.19798122730018308No Hit
CCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC418080.18434331420160027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA414640.18282652076289596No Hit
CCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG405230.17867738522271928No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA396500.17482808094368185No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC390910.17236329160578734Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA362850.15999084280054213Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA361990.15961164444086606No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA355210.15662215039597788No Hit
AATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA334270.1473891112661905No Hit
GCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG332580.14664394239659448No Hit
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA330900.14590318281025053No Hit
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA325610.1435706719699174No Hit
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA307370.13552813931818283No Hit
CCGCACCGTCGCTTAAAGTGACGGCATAATAATAAAAAAAAAAAAAAAGA297190.1310394889675985No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA295870.13045746357832824No Hit
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA282470.12454902402058464No Hit
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA280310.1235966188381424No Hit
CGCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG278360.12273680860399314No Hit
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC276890.12208864396594217Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG275120.12130820083032975No Hit
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC274810.1211715130495163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA269940.11902419210576917No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA267090.11776754637893565Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCT263710.11627720863974361TruSeq Adapter, Index 19 (97% over 38bp)
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC260620.11491474011486094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA259890.11459286243746147No Hit
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC258130.11381682858510112Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG247300.10904157482313373No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA243830.10751155353467326No Hit
CGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA240400.10599916937922098Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA237030.10451324092328099No Hit
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC235460.10382098345270954Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG233700.10304494960034918No Hit
CGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG229180.10105195357042374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)