FastQCFastQC Report
Fri 7 Jul 2023
SRR7235650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24711684
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3451541.3967239140804812No Hit
GGGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG1133780.45880321227804627No Hit
GCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG882290.3570335392764006No Hit
CCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG711410.2878840632633535No Hit
CGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG696730.2819435535028693No Hit
GCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG692730.2803248859932006No Hit
CCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG686480.2777957180093433No Hit
GGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG632910.2561177133861051No Hit
CCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG619730.2507842039417467No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA617490.24987775013633226No Hit
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA611630.2475064022346676No Hit
CCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA578260.23400266853525645No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA555610.22483696376175738Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA547530.22156725539222663Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG536980.21729801983547536No Hit
GCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG504650.2042151396885781No Hit
GGGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA502250.20324393918277686No Hit
CCGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG492250.1991972704086051No Hit
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA469870.19014082569200869No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA460440.18632481703796472No Hit
CGCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG452040.18292561526766044No Hit
GCGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG448000.18129076108289505No Hit
GCCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAA443350.17940906010290517No Hit
GGCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG438240.17734121235930342No Hit
CCCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAA432450.17499819113905793No Hit
CCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA430950.17439119082293217No Hit
CGCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAA430940.174387144154158No Hit
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA419610.1698022684330214No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA412910.1670910003543263Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA410320.16604291314181585No Hit
CGGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG409360.16565443293949533No Hit
GGGGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAA388770.15732234193347566No Hit
GCGGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA386280.1563147214087069No Hit
CGCTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA386100.15624188137077183No Hit
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA373690.15121996542202468No Hit
GGCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAA346000.14001473958634303No Hit
CCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA340980.1379833118617088No Hit
CCCTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA336940.13634845767694342No Hit
GCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA335520.13577383071101104No Hit
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA333080.13478644353011313No Hit
GGGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA332300.13447080336572773No Hit
GGCTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA332070.13437772998392178No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA331460.1341308831886973No Hit
GCCTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA327230.13241914229722265No Hit
GCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGA304690.1232979508802395No Hit
GCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA299280.12110870307341255No Hit
CGGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG288100.11658452738388854No Hit
AAACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA284840.11526531336350854No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269450.10903749012005819No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA263880.10678349561284452Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGAAAAAAAAAAA261740.10591750849517176No Hit
CGGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAAAAAAAAA253970.10277324685764029No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA248740.10065683908874846No Hit
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA248120.10040594562474982No Hit
CGCCCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG247760.10026026554887962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)