FastQCFastQC Report
Fri 7 Jul 2023
SRR7235651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22155307
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11371675.132707030419394No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1703860.7690527601355287Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1607510.7255643083618747Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC1520040.6860839256255848No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1279170.5773650529870789Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC959120.43290756476540815No Hit
CGGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC956480.43171597667321876No Hit
GCGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC872590.39385145960739787No Hit
CCCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC819470.36987526284334493No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA799470.36084808032675875Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC742990.33535531689991926No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA708510.3197924542413247Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG646840.2919571369514311No Hit
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC599020.27037314355427344Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA553550.24984984410281472Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC546190.246527840936711Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC528270.23843948540184975Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCG518340.2339574892823647No Hit
GGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC507240.22894740298565938No Hit
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA488320.22040768832496885No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA484150.2185255207702606No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA441860.1994375433389391Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC426540.19252272153123404No Hit
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA426240.19238731379348523Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA419580.18938126201546202No Hit
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA419090.18916009604380565No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA384900.17372812753170155Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA369750.1668900367753875No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA365150.1648137847965727No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA361060.1629677259719308Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCG347100.15666675257535362No Hit
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA341770.1542610084346834No Hit
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA330110.14899816102751362No Hit
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA320950.14486371143491714No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA318230.14363601461266143No Hit
AATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298860.1348931883453477No Hit
CCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC298630.13478937574640695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA297960.1344869651321013No Hit
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA296740.13393630699858955No Hit
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA285650.1289307342931425No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA276010.12457963232014795Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA274860.12406056932544425No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG273960.12365434611219786No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC273730.12355053351325712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA253790.11455043254422068No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250640.11312865129785835No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG249440.11258702034686316No Hit
GCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA232060.10474239873994974No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG227780.1028105816814003No Hit
GCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCG226990.10245400797199515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)