FastQCFastQC Report
Fri 7 Jul 2023
SRR7235652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20963508
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1769070.8438807092782372No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA716550.34180825079466665Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA696040.3320245829085475Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC594540.2836071138475488No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA489060.23329110757607935Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA442640.21114786704591618No Hit
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA436390.20816649579831772No Hit
GGGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG432920.20651123848165107No Hit
GGGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC387090.1846494393972612No Hit
CGGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC373370.17810473323453307No Hit
AAACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA367580.17534279091075788No Hit
CGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG366300.17473220607924972No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG364250.1737543163100374No Hit
GCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG351340.16759599586099808No Hit
CCCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG349890.16690431773155523No Hit
GCGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC337200.16085094155043134No Hit
GCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG336890.16070306553655048No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG331580.15817009252459083No Hit
CCCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC330490.15765014137900965No Hit
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA330260.15754042691709802No Hit
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA328560.15672949393775126No Hit
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA321320.1532758734845332Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG320320.15279885408491745No Hit
CGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCC304620.14530964951095016No Hit
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA298160.14222810418943244No Hit
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA295860.14113095957031618No Hit
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG290560.1386027567523527No Hit
GGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG276910.1320914419475977No Hit
GGGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG276780.13202942942564766No Hit
CCCGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG271170.1293533505938033No Hit
CCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAG267520.12761222978520578No Hit
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA261270.12463085853760736No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAGA259220.12365296876839507No Hit
CCGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG257730.12294220986296758No Hit
CGCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCG247250.11794304655499452No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA242890.11586324197266984Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG239690.11433677989389944No Hit
GCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA239290.11414597213405314No Hit
CGCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAGA236920.11301543615696381No Hit
GCGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG236660.11289141111306372No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA235040.1121186396856862Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA229920.10967630035965356No Hit
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA227480.10851237302459112No Hit
CGGTGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG215810.1029455566310753No Hit
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC210880.10059385099096964Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAGA210190.10026470760523477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)