FastQCFastQC Report
Fri 7 Jul 2023
SRR7235653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23817514
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1941730.8152530108725874No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1681860.706144226471327Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1533630.6439085120302648Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1121540.47088877537764856Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA758220.3183455670478455Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC656150.27549054867775036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC567970.23846737321115882Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA550140.23098128545237762Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC547810.23000301374862214Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA534920.22459102994544267Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA424430.17820079795061733No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA423410.1777725416682866No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA416600.17491330119507856Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA368620.1547684615615215Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAA350830.14729916816675326No Hit
GGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA325310.13658436392647863No Hit
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA325300.1365801653354754Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA315360.13240676587825245No Hit
GCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA298070.12514740203364633No Hit
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA298030.12513060766963338Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA290080.12179272782205566No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC287400.12066750543318669Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA269340.1130848500813309No Hit
CCCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAAAAA266220.11177488968831928No Hit
GGCCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAAA261760.10990231810087317No Hit
CCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC261440.10976796318876941Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA261150.10964620404967539Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAA260720.10946566463653595No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC255220.10715643958475263No Hit
GGCTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAA246420.10346167950189933No Hit
CCCTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAA244410.1026177627102476No Hit
CCGTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAA241360.10133719245425866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)